M. Aebi, N-linked protein glycosylation in the ER, Biochim. Biophys. Acta 2013 (1833) 2430-2437.Aebi, M. N-linked protein glycosylation in the ER. Biochimica et biophysica acta 1833, 2430-2437, doi: 10.1016/j.bbamcr.2013.04.001 (2013)....
N-linked protein glycosylation in the endoplasmic reticulum (ER) is a conserved two phase process in eukaryotic cells. It involves the assembly of an oligosaccharide on a lipid carrier, dolichylpyrophosphate and the transfer of the oligosaccharide to selected asparagine residues of polypeptides that hav...
The processing part of the N-glycosylation starts in the ER by trimming the glucoses (catalyzed by glucosidases I and II) and one mannose (catalyzed by α-mannosidase I). The residual glycoprotein intermediate is directed to the cis-Golgi where the processing pathway branches. A minor branch ...
Advanced protein glycosylation in diabetes and aging The SWISS-PROT protein sequence data bank contains at present nearly 75,000 entries, almost two thirds of which include the potential N-glycosylation conse... Brownlee,M.D,Michael - 《Ann Rev Med》 被引量: 1465发表: 1995年 Protein glycosylat...
Protein glycosylation can be categorized in two main types: a) N-linked glycosylation:It begins with the addition of a 14-sugar precursor to an asparagine amino acid. It contains glucose, mannose and n-acetylglucosamine molecules. This entity is then transferred to the ER lumen. The oligosaccha...
1.17.6 Glycosylation Glycosylphosphatidylinositol Anchor Addition In addition to signal sequence cleavage, disulfide bond formation and glycosylation glycosylphosphatidylinositol (GPI) anchor addition also takes place in the ER lumen. GPI-anchored proteins are cell-surface proteins that participate in transmembr...
Protein glycosylation has multiple functions in the cell. In the ER, glycosylation is used to monitor the status of protein folding, acting as a quality control mechanism to ensure that only properly folded proteins are trafficked to the Golgi. Sugar ...
in the ER and Golgi apparatus to form three main types of N-glycan chains: high mannose, heterozygous, and complex. N-linked glycosylation is essential for protein folding, stability, cellular localization, and multiple biological processes such as cell adhesion, proliferation, and signaling. In ...
We examined several key enzymes involved in protein N-glycosylation in both ER and Golgi apparatus. α-mannosidase from Jack Bean is a broad-specificity exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing α1–2, α1-3, and α1–6-linked mannose residues from oligosaccharides...
Infilamentous fungi, thesecretory proteinsare mannosylated by protein O-mannosyltransferase (PMT) starting in the endoplasmic reticulum (ER).98After leaving the ER the proteins are subsequently glycosylated in the Golgi apparatus to diversify the structure of the O-glycans. These glycosylations improve ...