This method of protein estimation is also known as the Lowry method because in 1951, Lowry et al. used this principle for the estimation of proteins in the biological samples. In this method, protein reacts with Folin phenol reagent to produce a blue color. The absorbance of this blue ...
Protein estimation with Pholin -Ciocalteau reagent We report that phosphorylated tyrosine is unable to react as a phenol with the Folin-Ciocalteau reagent in the commonly used protein estimation procedure ... O Lowry,N Rosebrough,A Farr,... 被引量: 14发表: 1951年 Investigation of the Folin-Ci...
Based on Lowry's Method of soluble protein estimation, the highest amount of protein was found in Button mushroom (2.35 mg/ml) followed with King oyster mushroom (2.24 mg/ml), Portabella Mushroom (2.07 mg/ml) and least amount of protein was found in Oyster mushroom (1.20 mg/ml). The ...
Interference caused by Tris buffer in the method of Lowry et al. (NAR 21, 4458) can be overcome by incorporating the same amount of Tris in the standards used. Distortion at <0.15 mM Tris was within tolerable limits. Sodium phosphate buffer, 40 mM, did not interfere. An automated Lowry ...
The Nitric Acid Method for Protein Estimation in Biological Samples Scott A. Boerner, Crescent R. Isham, Yean Kit Lee, Jennifer D. Tibodeau, Scott H. Kaufmann, Keith C. Bible Pages 35-45 Quantitation of Tryptophan in Proteins Alastair Aitken, Michèle P. Learmonth ...
Fig. 3. Estimation of protein mass per cell using two biochemical approaches and the proteomic ruler method. A, the histone proteomic ruler concept. The mass of cellular DNA is approximately equal to the protein mass of histones. Relating the histone MS signal to the total MS signal therefore...
Comparison of Classical Lowry, Modified Lowry and a Dye-Binding Assay for the Estimation of Protein in Allergen Extracts and Influence of Different Paramet... 8. The value of single factor studies as a preliminary to the synthetic method of investigation of such a process as biological decompositi...
Protein estimation in tissues containing high levels of lipid: Modifications to Lowry's method of protein determination A method to estimate protein in detergent-solubilized homogenates of lipid-rich biological samples (e.g., adipose tissue, myelin-enriched fractions of shee... GC Upreti,RA Ratcliff...
Protein estimation was performed in a small aliquot using the EZQ® Protein Quantitation Kit (Thermo Scientific, Waltham, MA, USA). 50 µg of protein extract were incubated with fluorophore-linked peptide substrate (LLVY-7-amino-4-methylcoumarin [AMC], from the same kit) for 60 min ...
Protein concentrations were determined by the Lowry method (48). In order to obtain the aKMT-SAH complex, aKMT copurified with SAH was incubated in 6 m guanidine-HCl, followed by incubation with SAH under renaturation conditions (49). Protein Crystallization, Data Collection, and Structure ...