Interpretation of protein electrophoresis.doi:10.1177/0004563217701505GAMMA globulinsTHERAPEUTIC use of monoclonal antibodiesELECTROPHORESISJialalIshwarlal.Annals of Clinical Biochemistry
Protein Electrophoresis, Immunofixation Electrophoresis Also Known As: Serum Protein Electrophoresis, Protein ELP, SPE, SPEP, Gel Electrophoresis, Capillary Electrophoresis, Immunosubtraction Electrophoresis, Urine Protein Electrophoresis, UPE, UPEP, IFE, CSF Protein Electrophoresis, Electrophoresis Board Approve...
Serum protein electrophoresis (SPE) is a common clinical laboratory test, mainly indicated for the diagnosis and follow-up of monoclonal gammopathies. A time-consuming and potentially subjective human expertise is required for SPE analysis to detect possible pitfalls and to provide a clinically ...
Protein Electrophoresis It is imperative for physicians who care forMMpatients to understand how to detectmonoclonal immunoglobulinin serum and urine. The gold standard for the diagnosis of MM has been proteinelectrophoresis. Even the gold standard test has limitations; it is unable to differentiate po...
Protein analysismay involveelectrophoresislinked to native or subunit activity determination,Western blotting, cofactor binding, or metal binding. Some analytic techniques use radiochemical or nonradioactive fluorescent imaging. The diagnostic test for carbohydrate-deficient glycoprotein syndromes isserum transferr...
Electrophoresis 14, 1382–1387 (1993). Article CAS Google Scholar Norman, M. E., Williams, P. & Illum, L. In vivo evaluation of protein adsorption to sterically stabilised colloidal carriers. J. Biomed. Mater. Res. 27, 8610866 (1993). Article Google Scholar Norman, M. E., Williams,...
C Electrophoresis mobility shift assay: 4D4 TCR was incubated at different ratios (1:1–7:1) with a fixed amount of PE and complex formation investigated in a 7.5% native PAGE gel. On separate wells PE or 4D4 were ran alone. Data is representative of 2 independent assays. D ...
The provided guidelines from study design to implementation to data interpretation serve as a reference for improving rigor and reproducibility of biomarker development studies.This is a preview of subscription content, access via your institution
The diagnosis could first be attempted by using less or non-invasive approaches (e.g. echocardiography, magnetic resonance imaging, imaging of reactive tags injected into circulation, PET/CT or serum and urine immunoelectrophoresis) before resorting to more invasive tissue biopsy [21, 23,24,25,26...
The protein complexes are then separated by electrophoresis on an SDS-PAGE gel to see how many individual proteins are associated in the complex. The identity of the associated proteins can be determined using such techniques as mass spectroscopy or protein sequencing. Sign in to download full-...