Thus, findings made showed varied protein profiles and intensity of protein spots detected on infected explants compared to the control, possibly as a result of explant co-cultivation with Agrobacterium. 展开 关键词: Agrobacterium cotyledonary nodes gel electrophoresis protein profiles soybean ...
This method can be used for different species, for different growth conditions, and it is likely suitable for the use in proteomic studies of other bacteria. Following protein extraction, a two-dimensional (2-D) gel electrophoresis proteomic technique is described to study global changes in the ...
Protein and amino acid composition of peanuts (Arachis hypogaea L.) inoculated with Aspergillus parasiticus Speare were compared with those of non-infected seeds during an 18-day test period to determine metabolic changes within this interrelationship. The levels of buffer-soluble proteins of infected ...
ComparisonofThreeMethodstoDetermine theWheyProteintoTotalProteinRatioinMilk 1 B.Miralles,B.Bartolome´,L.Amigo, andM.Ramos InstitutodeFermentacionesIndustriales(CSIC) JuandelaCierva,3,28006Madrid,Spain ABSTRACT Threemethodsforquantificationoftheratioofwhey proteinintotalprotein[capillaryelectrophoresis(CE),...
Cerebrospinal fluid Loading control Total protein stain Western blot 1. Introduction Western blotting (WB) is an antibody-based technique for the identification of protein targets transferred to a membrane following separation by polyacrylamide gel electrophoresis (PAGE). Refinements to the technique, inclu...
Optimizing the screening of alpha-1 antitrypsin deficiency using serum protein electrophoresis Anti-Ki/anti-PA28γ autoantibodies contribute to the HEp-2 indirect immunofluorescence nuclear speckled pattern Simultaneous quantification of tryptophan metabolites by liquid chromatography tandem mass spectrometry du...
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protein concentration. Despite the indirect nature of the test, there is a reliable correlation between the refractometer reading and total immunoglobulin concentration measured by RID. In healthy calves aserum total proteinof 5.5 g/dL or greater is associated with adequate transfer of passive ...
Fluorometric polyethyleneglycol-peptide hybrid substrates for quantitative assay of protein disulfide isomeraseAuthors: Christiansen C, St Hilaire PM, Winther JR.Journal: Anal Biochem (2004): 148 Assay of total homocysteine and other thiols by capillary electrophoresis and laser-induced fluorescence detection...
However, LOQs in the low nanogram/ml range or the detection of proteins expressed with a low copy number per cell require either immuno-enrichment of the target protein or fractionation of proteotypic peptides by cation exchange (24, 25) or off-gel electrophoresis (26). A generic strategy ...