Interpretation of protein electrophoresis.doi:10.1177/0004563217701505GAMMA globulinsTHERAPEUTIC use of monoclonal antibodiesELECTROPHORESISJialalIshwarlal.Annals of Clinical Biochemistry
(RIA). In these assays, rather than usinggel electrophoresis, the probing antibody is affixed to a substrate for detection of antigen in material passed over or through this substrate. As such, the specificity and sensitivity of antigen-antibody interactions needed for these tests are greater than...
of mass spectrometry and protein sequencing. Coverage includes: Peptide fragmentation and interpretation of product ion spectra Basic polyacrylamide gel electrophoresis Preparation of protein digests for sequencing experiments Mass spectrometric analysis using capillary liquid chromatography Techniques for protein ...
H. Colloidal carriers for intravenous drug targeting: plasma protein adsorption patterns on surface‐modified latex particles evaluated by two‐dimensional polyacrylamide gel electrophoresis. Electrophoresis 14, 1382–1387 (1993). Article CAS Google Scholar Norman, M. E., Williams, P. & Illum, L. ...
scattering; MALS multi-angle light scattering; MALLS multi-angle laser light scattering; CCL chemical cross-linking; FRET fluorescence resonance energy transfer; NMR nuclear magnetic resonance; SAXS small angle x-ray scattering; MS mass spectrometry; native-PAGE native polyacrylamide gel electrophoresis. ...
We earlier reported a simple specific test for detection of anti-ovarian antibodies in infertile women and identified number of specific molecular and cellular targets of which human heat shock protein 90-beta (HSP90 beta) was found to be the most immunodominant. The present study focuses on pred...
The protein bands observed on the electrophoresis gels were cut out, trypsin digested and the proteins were identified precisely using MALDI Tof-Tof, (MALDI: Matrix-assisted laser desorption/ionization; TOF: Mass spectrometer with Time of Flight (TOF) detector; Trypsin Gold from Promega, Madison, ...
The immunoprecipitated samples and purified extracellular NS1 proteins were analyzed by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and colloidal Coomassie Brilliant Blue G-250 staining. Protein bands corresponding to DENV NS1 were excised from the gels for further processing ...
Beads were washed three times with 1% NP-40 buffer and eluted by boiling in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) loading buffer. For immunoprecipitation, cell lysates were collected, quantified and were mixed with respective antibodies. For each co-immunoprecipitation, ...
Proteins were loaded and separated by electrophoresis on polyacrylamide gels (iD PAGE gel, 4–12%, Eurogentec, (Seraing, Belgium) and then transferred to nitrocellulose membranes (iBlot® 2NC Mini Stacks, Thermofisher Scientific, Waltham, MA, USA). Standard PageRulerTM Plus prestained protein ...