The invention provides a protein of PNGase F and the application of the protein as PNGase F in protein deglycosylation in glycan analysis. The protein has the polypeptide of an amino acid sequence showed as SEQ ID No: 2 or the polypeptide encoded by a nucleotide molecular sequence shown as ...
Although this point mutant had a significant leftshifted voltage for half-maximal activation over a range of calcium concentrations (from 200 nM to 30 μM), whether this is a result of deglycosylation per se or an effect of the mutation of Asn to Ala is not known. The latter is a ...
18,19,20,21. The changes in a cell’s proteomic profile are associated with biological phenomena; some changes can be predicted with several tools such as the Gene Ontology (GO) enrichment analysis22,23and pathway analysis
Glycosylation influences cell surface receptors to modulate conformational change, protein turnover rate, and intermolecular interactions and subsequently changes the structure and functions of target proteins. For example, asparagine-linked (N-linked) deglycosylation of iron transporter ZIP14 at N102 was ...
Lectin enrichment and deglycosylation in H2O18. The digested peptides were mixed with a lectin solution containing a combination of ConA, WGA, and RCA 120 (Sigma, USA) at ratio of 2:1 (protein to lectin, w/w). The mixtures were then transferred to new YM-30 filter units. After...
range. Global analysis of glycosylation changes can be expanded by dichromatic lectin-based profiling methods for rapidly categorizing glycan branching structure as well as deglycosylation studies employing exo- and endoglycosidases (1,2. The basic principles and methodologies for the multiplexed proteomics...
Structure-based, targeted deglycosylation of HIV-1 gp120 and effects on neutralization sensitivity and antibody recognition Virology., 313 (2003), pp. 387-400 View PDFView articleView in ScopusGoogle Scholar [80] Y. Li, B. Cleveland, I. Klots, B. Travis, B.A. Richardson, D. Anderson, ...
Glycan binding in theTatCel7A_CD thio-cellotrioside complex structure.aSuperposition of ligand binding in the SG3 structure (blue) with cellononaose in theTreCel7A Michaelis complex (4C4C; gray).bElectron density for the ligand at subsites − 6/− 5/− 4.cElectron density for the ...
In addition, the glycan chain appeared not to be required for the activation of Akt signaling in intestinal epithelial cells by Msp1. On the other hand, examination of different cell extracts showed that Msp1 is a glycosylated protein in the supernatant, but not in the cell wall and cytosol...
Glycosyltransferases (GTs) are enzymes that catalyze the formation of glycosidic bonds and hundreds of GTs have been identified so far in humans. Glycosyltransferase 8 domain-containing protein 1 (GLT8D1) has been associated with central nervous system d