The invention provides a protein of PNGase F and the application of the protein as PNGase F in protein deglycosylation in glycan analysis. The protein has the polypeptide of an amino acid sequence showed as SEQ ID No: 2 or the polypeptide encoded by a nucleotide molecular sequence shown as ...
Hatakeyama S,Amano M,Tobisawa Y,et al.SerumN-glycan alteration associated with renal cell carcinoma detected by high throughput glycan analysis[J].J Urol,2014,191(3):805-813.DOI: 10.1016/j.juro.2013.10.052. [76] Zhu HY,Liu MY,Yu HJ,et al.Glycopatterns of urinary protein as new pote...
Although this point mutant had a significant leftshifted voltage for half-maximal activation over a range of calcium concentrations (from 200 nM to 30 μM), whether this is a result of deglycosylation per se or an effect of the mutation of Asn to Ala is not known. The latter is a ...
(B) SDS-PAGE analysis of purified GLT8D1 stained with Coomassie Blue. The amount of protein was 3.5 µg. (C) Deglycosylation of GLT8D1. GLT8D1 without treatment (1), or incubated with PNGase F buffer (2), PNGase F (3), Endo H buffer (4) and Endo H (5) is shown. ...
the mixture of glycan, peptide, and glycopeptide fragments makes product ion spectra very complex, thus hindering the processing of the LC/MS/MS data of glycopeptides29,30. Although automatic analysis software has become available for data processing31,32, failure to unequivocally identify glycoprotein...
They can also be useful in determining the exact glycan chemistry required for interaction with a particular glycan-dependent antibody, as they will facilitate mass spectrometric glycan analysis due to a reduced background. In the current designs, ˜70–90% of the Asn residues at core gp120 ...
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Glycan binding in theTatCel7A_CD thio-cellotrioside complex structure.aSuperposition of ligand binding in the SG3 structure (blue) with cellononaose in theTreCel7A Michaelis complex (4C4C; gray).bElectron density for the ligand at subsites − 6/− 5/− 4.cElectron density for the ...
Misfolded glycoproteins are retrotranslocated to the cytosol and degraded by proteasomes. In this step, effective degradation is mediated by the deglycosylation activity of the cytosolic peptide N-glycanase (PNGase or NGLY1). The occurrence of non-glycosylated proteins in NGLY1 knockout cells under ...
The repeat units of the disaccharide [α1,3Xyl-β1,3GlcA] is termed matriglycan [5]. 3. Crucial enzymes of O-mannosylation: from structure to function Various enzymes have been involved in the process of O-mannosylation in mammalian cells. Most of them belong to intramembrane ...