Image generation in the HPA Cell Atlas In the HPA Cell Atlas, each target protein is imaged in three different cell lines selected based on messenger RNA expression data from a large panel of cell lines. A standard immunostaining protocol is applied in a 96-well format to stain the target ...
The approach taken here to generate a dynamic protein atlas of human cell division is generic; it can be applied to systematically map and mine dynamic protein localization networks that drive cell division in different cell types, and can be conceptually transferred to other cellular functions. ...
Candidate serological biomarkers for cancer identified from the secretomes of 23 cancer cell lines and the human protein atlas. Although cancer cell secretome profiling is a promising strategy used to identify potential body fluid-accessible cancer biomarkers, questions remain regar... CC Wu,CW Hsu,...
UHLéN M, BJöRLING E FAU - AGATON C, AGATON C FAU - SZIGYARTO C A-K, et al. A human protein atlas for normal and cancer tissues based on antibody proteomics. WANG C I, ALEKSEYENKO A A, LEROY G, et al. 2013. Chromatin proteins captured by ChIP-mass spectrometry are linked to ...
The program has generated protein expression profiles in human normal tissues with cell type-specific expression patterns, cancer and cell lines via an innovative immunohistochemistry-based approach. These profiles are accompanied by a large collection of high quality histological staining images, annotated...
2024年3月15日,嘉兴大学附属妇儿医院的车璇教授团队与同济大学高绍荣教授团队在Protein & Cell在线发表研究论文“Comprehensive transcriptional atlas of human adenomyosis deciphered by the integration of single-cell RNA-sequencing and spatial transcriptomics”。
More recently, with high quality datasets (see the section above), these findings have been confirmed in metazoan species (Alli Shaik et al., 2014, Vogel and Marcotte, 2012), between functionally different human cell lines (i.e., as different steady states) (Lundberg et al., 2010), and ...
An atlas of chaperone–protein interactions in Saccharomyces cerevisiae: implications to protein folding pathways in the cell Molecular chaperones are known to be involved in many cellular functions, however, a detailed and comprehensive overview of the interactions between chaper... Y Gong,Y Kakihara...
The mRNA for the receptor was detected in all pancreatic carcinomas, and growth of their derived cell lines was inhibited by antagonists blocking CCK2 (215, 216). Most of the neuropeptide receptors act through Gαq proteins, highlighting again the transforming potential of Gαq–GPCRs when ...
According to The Human Protein Atlas (www.proteinatlas.org), Lsm12 at the RNA level is ubiquitous and abundantly expressed in different tissues and cell lines. Given that Lsm12 is functionally critical for NAADP-evoked Ca2+ release in all three tested cell types (HEK293, SK-BR-3, and ...