The development of single-cell and spatial transcriptomics methods was instrumental in the conception of the Human Cell Atlas initiative, which aims to generate an integrated map of all cells across the human body. These technology advances are bringing increasing depth and resolution to maps of huma...
Here, we present the design and analysis of the results from the competition Human Protein Atlas – Single-Cell Classification hosted on the Kaggle platform. This represents a crowd-sourced competition to develop machine learning models trained on limited annotations to label single-cell protein ...
CELL type Atlas:跟Tissue Atlas前面类似,存在general information, Human protein atlas information, single cell types以及single cell tissue。另外也可以查阅在细胞系中表达情况。 Pathology Atlas:前面类似,包含general information, Human protein atlas information,增加了免疫组化信息,如数据可靠性,证据等级及相关抗体。
Mapping the functional impact of non-coding regulatory elements in primary T cells through single-cell CRISPR screens Celia Alda-Catalinas Ximena Ibarra-Soria Radu Rapiteanu Genome Biology(2024) Single-cell multi-omics map of human fetal blood in Down syndrome ...
Single-cell RNA Cell capture and library preparation The CD45+ cells were counted and measured for viability using Vi-Cell XR Cell Viability Analyzer (Beckman-Coulter). The barcoded Gel Beads were thawed, and the cDNA master mix was prepared using Chromium Single Cell 3’ v3 library kit (10x...
et al. A single-cell transcriptome atlas of human euploid and aneuploid blastocysts. Nat Genet 56, 1468–1481 (2024). https://doi.org/10.1038/s41588-024-01788-6 Download citation Received24 March 2023 Accepted06 May 2024 Published05 June 2024 Issue DateJuly 2024 DOIhttps://doi.org/10.1038...
we addressed this limitation by describing the developmental trajectories of different cell types that make up the human liver at single-cell resolution. These transcriptomic analyses revealed that sequential cell-to-cell interactions direct functional maturation of hepatocytes, with non-parenchymal cells pl...
Here we perform massively parallel single-cell RNA sequencing (scRNA-seq) of human retinas using two independent platforms, and report the first single-cell transcriptomic atlas of the human retina. Using a multi-resolution network-based analysis, we identify all major retinal cell types, and ...
While single-cell technologies have greatly advanced our comprehension of human brain cell types and functions, studies including large numbers of donors and multiple brain regions are needed to extend our understanding of brain cell heterogeneity. Integ
To address this gap, we integrated several single-cell ribonucleic acid sequencing datasets of the human small intestine (SI) to create an SI transcriptional atlas throughout the human life span, ranging from the first trimester to adulthood, with a focus on immune cells. Fetal SI displayed a ...