Here, we review the current progress made on endosomal escape of nucleic acid-based drugs, with a particular focus on RNA therapeutics (although some examples refer to DNA-based systems), mediated by peptide- and protein-based endosomal escape enhancers. This involves the prominent and widely used...
Using model proteins, we show that the spectra resulting from nanopore experiments and mass spectrometry share similar profiles, hence allowing protein fingerprinting. The intensity of individual peaks provides information on the concentration of individual peptides, indicating that this approach is ...
How do peptide bonds, polypeptides, and polypeptide chains relate to amino acids? What are the levels of protein structure? What is the difference between an enzyme and a substrate? What is the difference between a co-enzyme and a substrate?
Proteins are intimately involved in virtually everything that happens inside our cells and are infinitely more diverse and complex than carbohydrates and fats. Below are just a few examples of important bodily proteins: Enzymes (to run chemical reactions) Peptide hormones (example: insulin) Antibodies...
Once the DPC is degraded, a peptide is likely to remain cross-linked to DNA and it remains to be determined whether such peptide adducts can then be bypassed by the polymerase65 and/or are removed by other, as-yet unknown, mechanisms. Together, our findings reveal previously unrecognized ...
The adsorption of charged proteins on oppositely charged membranes has been studied by many groups, both experimentally and theoretically. The electrostatic binding of various peptides on lipid membranes was calculated and compared to experiment by Ben-Tal et al., 1996, Ben-Tal et al., 1997; Mur...
Integrated platform for proteome profiling with combination of microreversed phase based protein and peptide separation via online solvent exchange and pro... An online integrated platform for proteome profiling was established, with the combination of protein separation by microreversed phase liquid chromat...
Proteins were digested with trypsin, labelled using the eight-plex iTRAQ isobaric mass tags (ABSciex) and analyzed using tandem mass spectrometry on an LTQ Velos Orbitrap interfaced with an Eksigent 2D NanoLC as previously described [11], [30], [31], except mass tagged peptides were ...
et al. Human receptors patched and smoothened partially transduce hedgehog signal when expressed in Drosophila cells. J. Biol. Chem. 281, 28584–28595 (2006). PubMed Google Scholar Tu, C. et al. Optimization of search engines and postprocessing approaches to maximize peptide and protein ...
The human proteome dataset contains more than 20,000 proteins or peptides with lengths between 2 and 34,350 amino acids collected from UniProt (https://www.uniprot.org/uniprotkb/?facets=reviewed%3Atrue&query=proteome%3AUP000005640). In 2021, DeepMind released the AlphaFold2 model database, Alph...