To improve understanding of human profilaggrin processing to filaggrin, we produced seven monoclonal antibodies against epidermal filaggrin (AHF1-7). They were characterized on human epidermis by indirect immunofluorescence, immunogold labeling, and immunoblotting and found to be directed against seven ...
, 2009). The precise function of the C-terminal domain is unclear, but it is known to be required for the processing of profilaggrin to filaggrin. Loss-of-function mutations within FLG exon 3 result in a truncated profilaggrin molecule, which lacks the C terminus, resulting in an almost ...
We have recently identified a novel protein named hornerin, the structural features of which are most similar to those of profilaggrin, an essential protein for keratinization of epidermal tissues. In this study we examined the expression of hornerin compared with that of profilaggrin in various mou...
These studies suggest that human skin filaggrin, like rodent filaggrin, is synthesized as a high-Mr, phosphorylated, histidine-rich precursor (profilaggrin) that is processed via posttranslational modification to filaggrin. In human keratinocyte cultures a similar high-Mr precursor is present, but ...
Characterization of protease processing sites during conversion of rat profilaggrin to filaggrin. Biochemistry 1993;32: 10036-45.Resing KA, Johnson RS, Walsh KA: Characterization of protease processing sites during conversion of rat profilaggrin to filaggrin. Biochemistry 32:10036- 10045, ...
These findings demonstrate that flaky tail mice express an abnormal profilaggrin polypeptide that does not form normal keratohyalin F-granules and is not proteolytically processed to filaggrin. We propose that the absence of filaggrin, and in particular the hygroscopic, filaggrin-derived amino acids ...
PROBLEM TO BE SOLVED: To provide a novel antibody specific to a profilaggrin C-terminal domain that is important in keratinization of a keratin fiber of a granular layer of epidermis, a method for detecting the abnormality of a filaggrin gene where the antibody is used, and a kit for the ...
Profilaggrin requires both linker and filaggrin peptide sequences to form granules: implications for profilaggrin processing in vivo. J Invest Dermatol, 1999, 112: 843:852.M.K. Kuechle, C.D. Thulin, R.B. Presland, B.A. Dale, Profilaggrin requires both linker and filaggrin peptide sequence...
PROBLEM TO BE SOLVED: To provide a novel antibody specific to a profilaggrin C-terminal domain, a method for detecting the abnormality of a filaggrin gene using the antibody, and a kit for the detection.SOLUTION: There are provided an antibody specific to a C-terminal domain of a human ...
In upper granular layer cells, B-domain containing keratohyalin granules were in close apposition to the nucleus and, in some cells, appeared to be completely engulfed by the nucleus. In transition layer cells, B-domain staining was evident in the nucleus whereas filaggrin staining remained ...