, 2011). It is unclear as to how these observations may be attributable to filaggrin deficiency from our current understanding of filaggrin processing, and they may represent secondary effects. Filaggrin contributes to the protein–lipid cornified cell envelope, which replaces the plasma membrane of...
To investigate how DON affects HaCaT, human immortalized keratinocytes, we used CCK-8 assay and a quantitative real-time RT-PCR method to detect changes in the expression of tight junctions and skin cell regulatory proteins. The CCK-8 assay was performed to determine the growth inhibitory ...
The second aim of our studies was to correlate insolubility and granule formation of profilaggrin. It is known that profilaggrin undergoes both specific proteolysis and dephosphorylation during its conversion to filaggrin. Profilaggrin has previously been shown to become partially, but not completely, ...