Reverse transcription-polymerase chain reaction (RT-PCR) technique is extremely useful for sensitive detection of cancer cells, but it requires at least 8 h. If the precise information on the cancer spread based on RT-PCR is available during surgery, we can give the optimal therapy to each ...
PCR is a laboratory technique that replicates segments of DNA, creating millions or billions of copies. First, a reaction mixture containing a DNA template and supporting reagents is prepared. The thermocycler changes temperature to (1) melt the DNA so the double-stranded helix breaks into single...
AbstractCRISPR-Cas9 is a powerful gene editing technique that can induce mutations in a target gene of interest in almost any mammalian cell line. However, its practicality can be limited if target cell lines are difficult to transfect and do not proliferate. In the current study, we have ...
CRISPR-Cas9 is a powerful gene editing technique that can induce mutations in a target gene of interest in almost any mammalian cell line. However, its practicality can be limited if target cell lines are difficult to transfect and do not proliferate. In the current study, we have developed ...
Types of DNA Microarrays Spotted DNA arrays (“cDNA arrays”) Oligonucleotide arrays (Gene Chips) Requirements of DNA Microarray Technique Steps Involved in cDNA based Microarray Applications of DNA Microarray Advantages of DNA Microarray Disadvantages of DNA Microarray ...
This simple technique successfully recovered an average of 73% of the poliovirus added to clam homogenates at levels of 93 and 660 plaque-forming units per 100 g. Coxsackievirus B2 was isolated from clams exposed to raw sewage. 展开 关键词: angling animal viruses aquatic animals aquatic organisms...
. The sensitivity and specificity of this technique were compared to those of the usual diagnostic techniques. Sputum specimens were collected from 1,396 TB suspects attending the Rhodes Chest Clinic, Nairobi, Kenya. The specimens were analyzed for the presence of Mycobacterium tuberculosis by PCR; ...
Furthermore, the directed mutagenesis of mouse genes in embryonic stem cells has recently been carried out by resorting to a technique of “gene targetting” (Thomas et al., 1987; Thompson et al., 1989). In the first case, the mouse HPRT gene was mutated by insertion and replacement and...
双重RT-PCR方法 1. Detection of Avian Influenza Virus and Newcastle Disease Virus by Duplex RT-PCR Technique; 检测新城疫病毒和禽流感病毒的双重RT-PCR方法的建立 更多例句>> 补充资料:国际税收协定消除双重课税方法 国际税收协定消除双重课税方法 国际税收协定消除双重课税方法国际税收协定中,约束缔约国中的居住...
provide excellent performance in immunoassays and small molecule detection, also offers a highly sensitive platform for quantitation of nucleic acids, particularly when coupledwith an amplification technique such as Polymerase Chain Reaction (PCR... Richard Green,John Kenten - 《Biotechniques》 被引量: ...