The technique consists of two parts: The synthesis of cDNA (complementary DNA) from RNA by reverse transcription (RT) and The amplification of a specific cDNA by the polymerase chain reaction (PCR). RT-PCR has been used to measure viral load with HIV and may also be used with other RNA...
Describe the technique in detail. Explain the basic process of PCR. How does PCR work? What are the steps to be taken in the PCR Analysis Process from DNA Extraction through PCR reaction set up and process? What is PCR and what does it stand for? How does PCR work? (a) Describe DNA...
What are the steps to be taken in the PCR Analysis Process from DNA Extraction through PCR reaction set up and process? Explain with an illustration, the principles of the polymerase (PCR). Explain the process of PCR and the reagents that are needed to perform the reaction. Describe two pos...
Note:In order to remove DNase, which can destroy cDNA molecules in further qRT-PCR experiments, add 1ul of DNase inhibitor. DNA Library Preparation If the goal is to create a genomic DNA library, the first step is to extract genomic DNA (please see theprotocol for DNA extraction). If the...
Real-time PCR, also known asquantitative PCR or qPCR, is a technique for monitoring and quantifying PCR results in real-time by labeling DNA molecules with fluorescent dye. Reverse-Transcriptase(RT-PCR) converts RNA to DNA in the process of producing complementary DNA (cDNA). ...
. 5′ nuclease assays have the advantage of the precision that comes with using a sequence-specific, dual-labeled probe, and is the preferred technique for gene expression analysis. This article will focus on 5′ nuclease assay design and experimental setup considerations that will assist in ...
aThe polymerase chain reaction (PCR) is a technique widely used in molecular biology. It derives its name from DNA polymerase. PCR is used to amplify a piece of DNA by in vitro enzymatic replication. It uses repeated cycles, each of which consists of three steps: 聚合酶链式反应(PCR)是技术...
Polymerase chain reaction or PCR is a reaction that is utilised to amplify a gene or fragment of DNA of interest. It is done in vitro using a primer. This technique is used in labs to make billions of copies of the desired gene for research, diagnostic and therapeutic purposes....
The major technique involved in the Sanger sequencing method is polymerase chain reaction, or PCR. Like PCR, in vitro DNA replication takes place in the Sanger sequencing method, utilizing the reagents that are used in a PCR reaction. What are the 4 basic components of the Sanger sequencing re...
Gel electrophoresis is a common laboratory technique to isolate nucleic acids and proteins. Nucleic acid electrophoresis uses a gel matrix to separate DNA and RNA fragments based on size and molecular weight. Gel electrophoresis is vital i...