Sometimes called molecular photocopying, conventional polymerase chain reaction (PCR) is a technique used to amplify (replicate) trace amounts of DNA and RNA from a sample. A PCR thermal cycler is used to produce the large amounts required for research. The PCR process can be used for a ...
Describe the technique in detail. Explain the basic process of PCR. How does PCR work? What are the steps to be taken in the PCR Analysis Process from DNA Extraction through PCR reaction set up and process? What is PCR and what does it stand for? How does PCR work? (a) Describe DNA...
RNA Purification Kit A simple spin column technique for preparation of high quality, high‐purity intact total RNA. Find out moreDNA Library PreparationIf the goal is to create a genomic DNA library, the first step is to extract genomic DNA (please see the protocol for DNA extraction). If ...
. 5′ nuclease assays have the advantage of the precision that comes with using a sequence-specific, dual-labeled probe, and is the preferred technique for gene expression analysis. This article will focus on 5′ nuclease assay design and experimental setup considerations that will assist in ...
The major technique involved in the Sanger sequencing method is polymerase chain reaction, or PCR. Like PCR, in vitro DNA replication takes place in the Sanger sequencing method, utilizing the reagents that are used in a PCR reaction. What are the 4 basic components of the Sanger sequencing re...
DNA fingerprinting is a technique that involves creating a unique pattern of DNA from a crime scene sample and comparing it to potential suspects. DNA fingerprinting began with forensic uses but has more recently been used to determine paternity and ancestry, identify human remains, and compare two...
A portion of 25 g was taken from each foot, rinsed in 250 mL of 0.1% buffered peptone water and manually shaken for 1 min. The pour plate technique was used for TA and the most probable number (MPN) technique was used for TC and FC (USFDA, 1998). For Salmonella spp. and E. col...
The technique ‘Agarose Gel Electrophoresis’ reveals the progress of the restriction enzyme digestion. This technique involves running out the DNA on an agarose gel. On the application of current, the negatively charged DNA travels to the positive electrode and is separated out based on size. This...
What technique can be used especially for identification by extracting and identifying the base-pair pattern in an individual's DNA? How does one analyze DNA by the use of DNA fingerprinting? Describe the structure of bacterial DNA. Describe different steps of Sanger's method of nucleotides sequen...
Describe the technique in detail. B. What enzyme is used in this process and how is the enzyme used in PCR differ from others? C. What is the function of that enz How does DNA polymerase facilitate the PCR reaction? What is PCR, and what is required to make...