Wilusz J, Cheng A, Johnson C, Ford L. A Step-by-Step Procedure to Analyze the Efficacy of siRNA Using Real-Time PCR. In: Post-Tran- scriptional Gene Regulation: Humana Press, 2008;303-316.Cheng A, Johnson CL, Ford LP. A step-by-step procedure to analyze the efficacy of siRNA ...
Determine which RT-PCR procedure to use In performing RT-PCR, one-step and two-step methods are the two common approaches, each with its own advantages and disadvantages. One-step RT-PCR combines first-strand cDNA synthesis (RT) and subsequent PCR in a single reaction ...
Evaluating the Performance of the SureTect Salmonella Species PCR Assay to Detect Salmonella spp. from PPS Samples when Tetrathionate (TT) Broth and a Regrowth Step is Used as the Enrichment Procedure Quantitation, Detection, and Differentiation of Salmonella from Poultry Using Thermo Fisher Scientific...
The chapter describes a reliable step-by-step procedure to generate hairpin-linked DNA. It also provides a guide for efficient bisulfite conversion that is suitable for both conventional and hairpin bisulfite sequencing approaches. 展开 关键词: Ammonium sulfite monohydrate Bisulfite conversion Bisulfite ...
1993, " One step procedure of PCR-DNA extraction from paraffin-embedded materials by Chelex(R)-100 ", Acta Pathol. Jap. 43:790-791.Yoshirni N, Suzui M, Wang A, Kojima T, Moil H. One step procedure of PCR-DNA extraction from paraffin-embedded materialsby Chelex-100. Acta Pathol. ...
还有一种是REAL TIME-PCR 如果你方便的话，我就直接贴英语的了。不做翻译了。RT-PCR principles and procedure RT-PCR utilises a pair of primers, which are complementary to a defined sequence on each of the two strands of the cDNA. These primers are then extended by a DNA ...
PCRprinciplesandprocedure PCRmachine ApplicationofPCR Patentwars VariationsonthebasicPCRtechnique WhydidscientistsinventPCR? ThisisnecessarybecausemethodsusedforanalyzingDNA(determiningtheDNAbasepairsequence)requiremoreDNA. AparticularlyusefulfeatureofPCRisthatitallowstheamplificationprocesstobelimitedtospecificallytargeted...
高GC含量DNA的PCR详解 PCRAmplificationofHighlyGC-richRegions 高GC含量DNA的PCR扩增 ?1 高GC含量DNA序列扩增困难 生物中广泛存在高GC含量DNA序列,扩增的DNA有的含70%-80%高GC,常规PCR条件下,难扩增,原因:易形成复杂的二级结构,防止DNA模板变性,DNA聚合酶难以结合.2 高GC含量DNA序列扩增常遇到的问题 1.常规PCR....
In this study, a one-step, single tube, duplex polymerase chain reaction (PCR) procedure was developed to allow rapid, specific, and sensitive identification of B. tabaci MEAM1 and F. occidentalis in predator guts. The system and conditions used for the duplex PCR were optimized. The species...
-ggcagccaagccagcaccc-3′4. To construct binary vectors harboring two targets, starting with an ‘A’ and a ‘G’, respectively:5. Order the primers from any qualified company.step B. Prepare PCR templates1. Linearize the donor vectors with EcoRV.2. Recycle the two fragments produced by ...