What is PCR? What is required for PCR? What are the 4 steps of PCR? What are the PCR machine steps? What is the temperature used for the denature step? What happens during the annealing step of PCR? What is the temperature used for the extension step? What is the PCR temperature flow...
when the intron must release products of the first reaction and recruit substrates of the second splicing event. Biochemical and structural studies suggest that, after the first step of splicing, the intron rearranges at the K1-binding site,...
A PCR process for DNA amplification with thermocyclisation of the corresponding reagents is disclosed, in which total integration of all substances and process steps is achieved in a closed, single-use unit (so-called cartridge) in which the reagents are stored in a storage-stable form at room...
For each analysis, the percentage of variance explained by the first two dimensions is indicated in parenthesis. (a) MCA of all putative EPS-related proteins included in this study. Two major groups, separated by the first and second components, are observed. The larger group is encircled in ...
This accumulation is most likely explained by a lack of fusion between autophagosomes and endolysosomal compartments during basal autophagy. Alternatively, it could be an indirect effect of upregulation of proautophagic signaling or a failure in lysosome biogenesis (Figure 4K). We favor the first ...
A canonical correspondence analysis (CCA) based on the vegan package61was used to investigate the variations in OTU data under the constraints of our set of environmental variables. Significant variables (i.e., variables that significantly explained changes in community composition) in our data set ...
The differences in phenotype can most likely be explained by technical differences between the two approaches. The events of muscle patterning, which are controlled, in part, through the activity of Tbx5 in ICT/MCT, occur during a relatively narrow time window, around E11.5–12.5. Because there...
The zonal asymmetry might be explained by regional differences in the movement of Southern Ocean fronts that are less constrained by sea floor topography in the Indo-Pacific sector16. othuirsDAisuAraiIgnWagintrheaeccocYorDdmaptthaPenrSiee7di5s/b1eyv0i4ad-ne1ninpccoeriinenatsstihtnoegaiicn...
The telomeric DNA sequence (TTAGGG)n was also used as a probe in males of the karyomorph D. This probe was generated by PCR (PCR DIG-Probe Synthesis Kit; Roche) in the absence of template using (TTAGGG)5 and (CCCTAA)5 as primers (Ijdo et al., 1991). Fluorescent in situ ...
PCR polymerase chain reaction LTR long terminal repeat The retroviral gag gene encodes the major structural proteins of HIV-11 particles. The p55 gag precursor is cleaved by the viral protease in different proteins: the matrix MAp17, the capsid CAp24, the p6 gag, the nucleocapsid NCp7 associate...