PCR primerSTRSNPThe polymerase chain reaction (PCR) is a popular method to copy DNA in vitro. Its invention revolutionized fields ranging from clinical medicine to anthropology, molecular biology, and forensic biology. The method employs one of many available heat-stable DNA polymerases in a ...
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David P.Clark,Nanette J.Pazdernik, inMolecular Biology (Second Edition), 2013 Sequence added to primers or probes that may be used for binding to a support or may incorporate convenient restriction sites, primerbinding sitesfor future manipulations, orprimer binding sitesfor subsequent PCR reaction...
Real-time PCR detection chemistry E. Navarro, ... J. Solera, in Clinica Chimica Acta, 2015 2.2.1 Primer-probes Primer-probes are oligonucleotides that combine a primer and probe in a single molecule. They can be classified into three groups: Harpins, Cyclicons and Angler® primer-probes....
Jiaxuan Li, Guangcheng Han, Xiao Tian, Dan Liang*, Peng Zhang*. 2023. UPrimer: A Clade-Specific Primer Design Program Based on Nested PCR Strategy and Its Applications in Amplicon Capture Phylogenomics. Molecular Biology and Evolution. DOI: 10.1093/molbev/msad230 ...
One major challenge in the design of highly multiplexed PCR primer sets is the large number of potential primer dimer species that grows quadratically with the number of primers to be designed. Simultaneously, there are exponentially many choices for multiplex primer sequence selection, resulting in...
[Methods in Molecular Biology] PCR Primer Design Volume 1275 || Rapid and Simple Method of qPCR Primer Design Quantitative real-time polymerase chain reaction (qPCR) is a powerful tool for analysis and quantification of gene expression. It is advantageous compared ... Basu,Chhandak - Springer ...
Precise determination of mitochondrial DNA copy number in human skeletal and cardiac muscle by a PCR-based assay: lack of change of copy number with age. Nucleic Acids Res. 31, e61 (2003). Article Google Scholar Chinnery, P. F. & Turnbull, D. M. Mitochondrial DNA and disease. Lancet ...
Quantitation of DNA can play a primary role in forensic investigation and analysis. Biological samples may often be extracted from the crime scene, and profiling can be done in laboratories with the help of the right kits. The amplification of the samples as well as the use of agarose gel el...
et al. Role of nanoscale antigen organization on B-cell activation probed using DNA origami. Nat. Nanotechnol. 15, 716–723 (2020). ADS Google Scholar Surana, S., Shenoy, A. R. & Krishnan, Y. Designing DNA nanodevices for compatibility with the immune system of higher organisms. Nat. ...