What is the annealing step of a PCR and what factor(s) influence it? Why do one have to lower the annealing temperature for a 10-base primer in PCR? What are the two factors that contributed to the annealing temperature of a primer (in PCR)? What happens...
Explain how PCR is used to amplify DNA sequences. Why do we extract RNA and then convert it into cDNA to put in the PCR instead of just extracting DNA? Why do you need to use a buffer while studying DNA by electrophoresis? Why doesn't RNA polymerase need a primer?
During PCR and cycle sequencing, the DNA is first denatured (the double-stranded DNA template becomes single-stranded DNA). A subsequent annealing step allows for hybridization of the oligonucleotide primer close to the sequence of interest. In the extension...
What are primers made of in DNA replication? How do bases bond in single-stranded DNA? What are the nucleotides involved in DNA synthesis? What is D2-D3 ribosomal DNA? What does ribosomal DNA do? Explore our homework questions and answers library ...
Efficiency of Real-Time PCR What is multiplexing? Taq Talk Video Series Advantages of multiplexing Duplexing Three or more genes? Factors that affect the reliability of multiplex PCR assays General considerations Primer limitation Validation of multiplexing reactions In mu...
The first step in a PCR cycle is to heat the sample to 95°C. What is the purpose of heating the sample?A.To separate the two strands of DNAB.To allow the primer to anneal to the templateC.To produce the optimal temperature for Taq dna polymerase to workD.To produce the optimal ...
What is PCR and what does it stand for? How does PCR work? What is DNA replication and its relation to PCR? What is the role of the DNA primers in PCR? What does the PCR process entail? A. creates new DNA B. destroys DNA C. changes DNA D. copies existing ...
Also Read:How Does DNA Replication Occur? What Are The Enzymes Involved? What Are The Components Of A PCR Reaction? The key components of a PCR reaction are the template DNA, primers, nucleotides, and heat-stable DNA polymerase. Let’s briefly learn about each of these components. ...
How does RNA polymerase generate RNA without a primer? Why do we use DNA primer for PCR but RNA primer for DNA polymerase in cells? What is the role of DNA polymerase I? Polymerase chain reaction (PCR) is used to amplify specific DNA regions. What components are not needed for t...
What is the function of RNA primer during protein synthesis? What does DNA polymerase do in PCR? What role do genes play in protein synthesis? What is the function of rRNA ribosomes in protein synthesis? What is the role of DNA electrophoresis in DNA fingerprinting?