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of, relating to, or being the amino acid sequence in proteins primary protein structure resulting from the substitution of one of two or more atoms or groups in a moleculeespecially: being or characterized by a carbon atom having a bond to only one other carbon atom ...
Molecular Sequence DataOpen Reading FramesPurified alanine carrier proteins were cleaved into peptides either chemically after solubilization in 1,1,1,3,3,3-hexafluoro-2-propanol or proteolytically with lysylendopeptidase. From the amino acid sequence analyses of these peptides, we synthesized a DNA ...
output is reinjected into its input as illustrated in Fig.4. The output of the decoder is initialized with a beginning-of-sequence (BOS) token as well as with a prior about the binder amino acid sequence that consists of its first\(\textit{n}\)amino acids. The Bayesian prior is employe...
Purified alanine carrier proteins were cleaved into peptides either chemically after solubilization in 1,1,1,3,3,3-hexafluoro-2-propanol or proteolytically with lysylendopeptidase. From the amino acid sequence analyses of these peptides, we synthesized a DNA probe and utilized it for successful clon...
We used the maximum core similarity of 1.0 to exactly match the highest conserved bases of a matrix in the sequence. The threshold for the matrix similarity was set to 0.9 to minimize false positives. Hemiparkinsonian animal models For the analysis of neuronal PC in 6-hydroxydopamine-HCl (6-...
First, it is composed of repeating sequences of amino acids, both with and without interruptions. Next, within those repeating sequences, proline and hydroxyproline occupy the X and Y positions, respectively. Finally, its right-handed triple helix structure is formed from three left-handed ...
After incubation, 16 µL of 1% (v/v) ammonia was added to quench the reaction, followed by the addition of 8 µL formic acid. After vortexing, heavy (CD2O)- and light (CH2O)-labeled samples were combined at a ratio of 1:1 (v/v) to generate duplex dimethyl-labeled samples...
Bioorthogonal non-canonical amino acid tagging (BONCAT) MoDCs were cultured as described above. On day 7, medium was changed and medium without IL-4 and Gm-CSF was added with the appropriate DON and LPS supplementations. After 24 h, 48 h, or 72 h, medium cells were centrifuged (3 min...
Protein concentrations were determined with the Lowry method and cysteines (cysteic acid) determined by amino acid analysis (Model L8800, Hitachi High Technologies America, Pleasanton, CA). One aliquot of each sample (100 μg protein) was treated with 6 mM ascorbate (Asc+) to reduce the...