Reverse Transcribe RNA into cDNA Step 3 Set Up & Run qPCR Reactions Life Technologies offers a variety of kits that preserve small RNAs such as miRNAs. While it is not essential to use them due to the larger size of primary miRNA transcripts, these kits ...
To design the forward qPCR primer, please follow the guidelines on p.13 of the manual. What endogenous control gene can I use NCode™ SYBR® GreenER™ system? When considering endogenous controls suit...
Western blot和RT-QPCR结果表明,有两个siRNAs(#1和#3)有效抑制miPEP133mRNA的表达,显著降低抗miPEP133抗体检测到的15 kDa蛋白条带的强度(图1f和g)我们还分别产生了两个CRISPR/Cas9介导的miPEP133和MUT1 和MUT2.他们在miPEP133中缺失的ORF...
TaqMan® Pri-miRNA Assays采用了与所有Applied Biosystems® TaqMan® Gene Expression Assays相同的简单方便流程。无论是两步法实时定量PCR(RT-qPCR)步骤的可靠性,一步法RT-qPCR步骤的便利性,还是快速循环的速度,Applied Biosystems都提供了经过验证的试剂盒,支持这些不同的分析流程。在定量前必须确保样品是不含DN...
i, RT–qPCR analysis of pri-miRNAs in single and double mutants relative to Col-0. Total RNA was prepared from a pool of 21-day-old plants; EF-1α serves as a loading control. The relative signals of pri-miRNAs were normalized to those of wild-type Col-0, where the ratio was ...
HYL1是一种双链RNA结合蛋白,在miRNA生物形成中发挥重要作用。因此,作者利用RIP (HYL1-RIP)和RT-qPCR技术研究了WT和mta突变植株中HYL1与pri-miRNAs的结合。作者发现,与WT相比,在mta突变体的HYL1-IP样本中,检测到的10个pri-miRNAs中有7个明显较少(图3B)。
•Fast, simple, and scalable – two-step RT-qPCR provides high-quality results •A new perspective – measure miRNA expression at the gene level TaqMan® Pri-miRNA Assays offer the same high sensitivity, superior specificity, and wide linear dynamic range for which all TaqMan® Genomic...
•Fast, simple, and scalable – two-step RT-qPCR provides high-quality results •A new perspective – measure miRNA expression at the gene level TaqMan® Pri-miRNA Assays offer the same high sensitivity, superior specificity, and wide linear dynamic range for which all TaqMan® Genomic...
We recommend using the NCode™ VILO™ miRNA cDNA Synthesis Kit to generate cDNA. Multiple peaks in your melt curve could be from several sources: Contaminating gDNA—Make sure to DNase-treat your sample before...
We recommend using the NCode™ VILO™ miRNA cDNA Synthesis Kit to generate cDNA. Multiple peaks in your melt curve could be from several sources: Contaminating gDNA—Make sure to DNase-treat your sample ...