Set Up & Run qPCR Reactions Life Technologies offers a variety of kits that preserve small RNAs such as miRNAs. While it is not essential to use them due to the larger size of primary miRNA transcripts, these kits do enable both pri-miRNA and mature miR...
To design the forward qPCR primer, please follow the guidelines on p.13 of the manual. What endogenous control gene can I use NCode™ SYBR® GreenER™ system? When considering endogenous control...
虽然敲除DCL1会导致胚胎死亡,但HYL1的功能缺失突变体仍然存在,这表明HYL1并不是必需的。先前的研究表明,HYL1形成同源二聚体,可能结合前体RNA的miRNA/miRNA*(*,信使链)区域,提高DCL1介导加工的效率和准确性。然而,其具体的作用机制以及HYL1是否具有其他生物学功能尚不清楚。 2020年7月7日 复旦大学任国栋教授团队 ...
由于分析灵敏度高,TaqMan® Pri-miRNA Assays只需要极少的样品投入:低至1 ng的总RNA已足够定量中等至高表达miRNA位点的表达。 与miRNA研究领域的顶尖研究人员合作,我们展示了分析在分辨包含密切相关的miRNA茎环序列的不同基因座上的能力(图4)。这对于它们在研究基因调控这个重要方面的应用很关键。 图4. TaqMan®...
MiPEP133是由miR-34a的pri-miRNA中的一个开放阅读框架编码的微蛋白 MIR-34a由位于染色体1p远端区域的MIR34AHG基因编码。我们在MIR34A前体序列区域(图1a)附近发现了一个402bp的ORF。我们将ORF克隆到质粒载体上,转染HEK293细胞。利用SDS-PAGE和...
b, Re-analysis of previously published microarray data sets for chr2 mutants56,57,58 to assess the expression of numerous canonical genes involved in the miRNA pathways. c, d, RT–PCR (c) and RT−qPCR (d) show that the expression levels of the genes involved in the miRNA pathway are...
b Venn diagram showing miR-221-3p as the common differentially expressed miRNA between BC and normal breast tissue samples. c Expression box of miR-221-3p by the GSE37527 (left), GSE58027 (right) and GSE57897 (middle) datasets. d The expression of miR-221-3p was determined by RT-qPCR ...
First, the role of miRNA-encoded peptides in hypertension has not been reported. For the first time, we explored the role of miPEP31 in a hypertensive mouse model, and point mutant mice were constructed for validation. Moreover, the mechanism of miPEP31 treatment in hypertensive mice was ...
To parse out the role of METTL3 in ovarian carcinoma, we first detected the mRNA and protein expression of METTL3 in ovarian cancer tissues and adjacent normal tissues of 64 patients with ovarian cancer by RT-qPCR, immunohistochemistry, and Western blot analysis. The results showed that the mRN...
该平台利用了298个引物对,特别用于拟南芥pri-miRNA定量RT-qPCR。在检测的298个pri-miRNAs中,作者的样本中有68个pri-miRNAs未检测到,剩下230个有待进一步分析。RT-qPCR结果显示,85个pri-miRNAs的积累变化(WT vs. mta)具有统计学意义(P≤0.05,n = 3);其中56个(约为66%)被上调,29个被下调(图1C)。将RT-...