Androvic, P.; Valihrach, L.; Elling, J.; Sjoback, R.; Kubista, M. Two-tailed RT-qPCR: A novel method for highly accurate miRNA quantification. Nucleic Acids Res. 2017, 45, e144. [CrossRef] [PubMed]Androvic, P., Valihrach, L., Elling, J., Sjoback, R., and Kubista, M. ...
针对 miRNA 序列短小的 特点,开发出了多种检测方法,其中 miRNA qPCR 定量检测是应用最广泛的方法.就 miRNA 抽提,miRNA qPCR 定量检测原理及 miRNA qPCR 定量检测流程包括引物设计,反应体系设置,内参基因筛选等方面进行了深入探讨,并对 miRNA 定量检测研究方法 的发展趋势进行了展望,以期为研究者进行 miRNA qPCR 定量...
图 茎环法逆转录原理 (图源:Varkonyi-Gasic, E. , Wu, R. , Wood, M. , Walton, E. F. , & Hellens, R. P. . (2007). Protocol: a highly sensitive rt-pcr method for detection and quantification of micrornas. Plant Methods, 3(1), 12-...
Type: miRNA RT-qPCR Catalog Number: EZB-miProbe-R2-S/EZB-miProbe-R2 Pack Size: 200/500 Rxns Price: 250/500 USD Promotion: 200/400 USD (47 Customers Bought) MANUAL Introduction The EZBioscience® EZ-Probe qPCR Master Mix for microRNA(ROX2 plus)uses a specially modified Taq DNA polymer...
This product is a special premix for miRNA quantitative reaction using SYBR® Green I chimeric fluorescence method. Because miRNA sequences are short and miRNA sequences of the same family are often highly similar, the specificity requirements for quantification are extremely high. This product is ...
From sample to answer, learn about methods for optimal detection of miRNAs from serum and plasma miRNA detection and quantification method Although a variety of qPCR-based analysis solutions are currently available to quantify miRNAs, they may lack either sensitivity or specificity, have...
寡核苷酸药物主要包括小干扰RNA(siRNA)、反义寡核苷酸(ASO)、微小RNA(miRNA)、小激活RNA(saRNA)、核酸适配体(aptamer)和转录因子诱饵(decoy)、核酶(ribozyme)等。其中ASO和siRNA类型的寡核苷酸药物应用广泛。 寡核苷酸药物是由人工化学合成的寡核苷酸单链或双链组成的一类药物,一般由19-25个核苷酸组成,通过碱基互补配...
qPCR技术以其独特的技术特征越来越多的被应用到寡核苷酸药物的药代和药效动力学生物分析中。 寡核苷酸药的分类与作用机制 寡核苷酸药物主要包括小干扰RNA(siRNA)、反义寡核苷酸(ASO)、微小RNA(miRNA)、小激活RNA(saRNA)、核酸适配体(aptamer)...
Protocol: a highly sensitive rt-pcr method for detection and quantification of micrornas. Plant Methods, 3(1), 12-12.) 由于miRNA在20bp左右,无法根据其设计引物进行检测,可以先将其延长,也就是先加一个环状片段,这样就使得原来20bp左右的延伸为80bp左右了,然后就可以根据这个80bp片段设计引物。茎环法逆...
（SNP全称SingleNucleotidePolymorphisms单核苷酸多态性，是指在基因组上单个核苷酸的变异,包括置换、颠换、缺失和插入。SNP是第三代遗传标志,人体许多表型差异、对药物或疾病的易感性等等都可能与SNP有关）。Page7 QPCR应用于miRNA的定量 miRNA: