A lack of equal rights for potatoes led to a disease spreading between them and endangered the tradition of forking the potato in Ireland. Fortunately, other areas of the world maintained their healthy, diverse potatoes, and so the tradition lives on. You too can continue this tradition of fo...
A lack of equal rights for potatoes led to a disease spreading between them and endangered the tradition of forking the potato in Ireland. Fortunately, other areas of the world maintained their healthy, diverse potatoes, and so the tradition lives on. You too can continue this tradition of fo...
Genome-wide approach to identify quantitative trait loci for drought tolerance in tetraploid potato (Solanum tuberosumL.).Int. J. Mol. Sci.22, 6123 (2021). Michelmore, R. W., Paran, I. & Kesseli, R. V. Identification of markers linked to disease-resistance genes by bulked segregant analy...
P. infestans isolates T30-4 and 88069 were used for the disease test and were maintained on rye sucrose agar (RSA) medium in an 18 °C incubator. To induce zoospores, ice-cold water was added to the plates after 10–14 days. The plates were then incubated at 4 °C for 1–2...
RNA isolation and detection Total RNA was isolated from the above samples using TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer’s instructions, the amount of plant tissue for RNA extraction was 300 mg. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN...
Detection of DRs Re-sequencing reads of 1034 individuals of C88 S1 population were mapped to C88.v1, using BWA-MEM, and the normalized read numbers in a 100-kb window were calculated to genotype the population, using the method described in section “unitigs and HiFi reads phasing.” Ever...
PVDP: A portable open source pipeline for detection of plant viruses in RNAseq data. A case study on potato viruses in Antioquia (Colombia)BioinformaticsHigh-throughput sequencingNext-generation sequencingPlant virusesViromeHigh-throughput sequencing methods are becoming an essential tool in disease ...
The detection of potato wart disease on Prince Edward Island during the 2000 growing season resulted in an estimated $30 million loss to the island’s economy in the first year. 3.4 Pathogen Populations and Distribution Potato wart disease appears to have arisen in the potato-growing area of ...
Over the past years, considerable success has been made in redomesticating potato from a clonally propagated, tetraploid crop into a seed-propagated, diploid crop to increase reproduction rate, decrease costs in storage and transportation and improve disease control2,33,34,35. However, the random ...
Potato (Solanum tuberosum L.) is the most important tuber crop worldwide. Efforts are underway to transform the crop from a clonally propagated tetraploid into a seed-propagated, inbred-line-based hybrid, but this process requires a better understanding