linear polyubiquitin chainLUBACNEMONF│蔅ubiquitinThe ubiquitin-conjugation system regulates a vast range of biological phenomena by affecting protein function mostly through polyubiquitin conjugation. The type of polyubiquitin chain that is generated seems to determine how conjugated proteins are regulated, ...
Since different ubiquitin chain linkages exert distinctive functions, we next investigated the type of polyubiquitination mediated by USP18. We found that USP18 promoted the K63-linked rather than K48-linked polyubiquitination of MAVS, which was supported by several scenarios. First, overexpression of...
2Altmetric Metrics Abstract The extracellular-signal-regulated kinases ERK1 and ERK2 (hereafter ERK1/2) represent the foremost mitogenic pathway in mammalian cells, and their dysregulation drives tumorigenesis and confers therapeutic resistance. ERK1/2 are known to be activated by MAPK/ERK kinase (ME...
In most cases, TRIP12 as an E3 ligase directly attaches ubiquitin chain to the substrates and initiates proteasome-mediated degradation [17, 34]. This E3 ligase may inhibit EMT process in breast cancer as reported before [35]. In this study, we reveal a new and distinct role of TRIP12 ...
Several DUBs have been identified to regulate the Nanog protein stability. For example, USP21 was reported to interact with Nanog protein in embryonic stem cells [150–152]. USP21 deubiquitylated the ubiquitin chain of Nanog in a K48-linkage dependent manner [152]. ...
It has been reported that TRAF6 E3 ligase mainly promotes lysine (K) 63-linked polyubiquitin chain assembly (Chen, 2005). To investigate whether K63 or other ubiquitin chain linkage types such as K48 and K29 were used for ATG9A ubiquitination by TRAF6, we set up a ubiquitination assay with...
In most cases, TRIP12 as an E3 ligase directly attaches ubiquitin chain to the sub- strates and initiates proteasome-mediated degradation [17, 34]. This E3 ligase may inhibit EMT process in breast cancer as reported before [35]. In this study, we reveal a new and distinct role of TRIP...
Antigens internalized by the mannose receptor (MR), an endocytic receptor that targets its ligands specifically toward cross-presentation, were translocated into the cytosol only after attachment of a Iysin48-linked polyubiquitin chain to the cytosolic region of the MR. Furthermore, we identify TSG...
Conjugation of polyubiquitin chain to a target protein has initially been characterized as a signal for protein degradation 12 , which still appears to be a dominant role. However, polyubiquitination can trigger multiple functions, depending on which lysine residue (K) in ...
Protein ubiquitination involves the attachment of various ubiquitin chain types. RNF6 has been reported to induce K6/K27- and K48-linked polyubiquitination [2, 18]. To determine the specific type of ubiquitin chain linkage to GR mediated by RNF6, we screened a series of mutant ubiquitin plasmi...