In this study, we show that Aurora A polyubiquitinates OLA1, targeting it for proteasomal degradation, thereby downregulating centrosomal OLA1 and the recruitment of PCM proteins in G2 phase. NIMA-related kinase 2 (NEK2), which regulates centrosomes in G2 phase,27 promoted OLA1 polyubiquitinatio...
Fig. 3. Disruption of the PS1 CUE domain impairs binding to K63-linked polyubiquitin. (A) HEK293T cells expressing pcDNA3.1 (Vector), wild type PS1, PS1ΔCUE, PS1F283A/P284A or PS1V309A/S310A were lysed under stringent denaturing conditions, immunopurified with an anti-PS1 antibody and...
采用RACE(rapid amplification of cDNA ends)技术获得铁皮石斛(Dendrobium officinale Kimura et Migo)Polyubiquitin1(DoUb1),该基因全长1985 bp,开放阅读框为1371 bp,编码457个氨基酸残基的亲水性蛋白,分子质量为51 179.7 ku,理论等电点p I为7.76;亚细胞定位分析表明该基因产物主要在细胞质或线粒体基质中发挥作用.Do...
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Catalyzed by a cascade of E1, E2, and E3 enzymes, the ∼8-kDa protein ubiquitin is conjugated to protein substrates as a single moiety (monoubiquitin), as polyubiquitin chains linked through one of seven internal lysine residues of ubiquitin (Lys-6, Lys-11, Lys-27, Lys-29, Lys-33, ...
Moreover, the largest proteasomal receptor, Rpn1, contains one known binding site for polyubiquitin and shuttle proteins, although several studies have recently proposed the existence of an additional uncharacterized site. Here, using a combination of NMR spectroscopy, photocrosslinking, mass spectrometry...
1 and 2). Attachment of polyubiquitin chain(s) to a protein frequently serves to target the modified protein for proteolysis by the 26 S proteasome. The ubiquitin molecules in these chains are most often linked to one another by isopeptide bonds between the C terminus of one ubiquitin and ...
We find that TRAF6, a RING domain protein, functions together with Ubc13/Uev1A to catalyze the synthesis of unique polyubiquitin chains linked through lysine-63 (K63) of ubiquitin. Blockade of this polyubiquitin chain synthesis, but not inhibition of the proteasome, prevents the activation of ...
Tsukiyama, A. Nakajima, S. Maruyama, M. Watanabe, T. Koike, S. Hatakeyama Involvement of Ymer in suppression of NF-κB activation by regulated interaction with lysine-63-linked polyubiquitin chain Biochim. Biophys. Acta, 1783 (2008), pp. 826-837 View PDFView articleView in ScopusGoogle ...
In this work, we investigate T.th-ubl5, a UbL present in a polyubiquitin locus of Tetrahymena thermophila, which is integral to an ADP-ribosyl transferase protein. Only one other co-occurrence of these two domains within the same protein has been reported. Methods NMR, multiple sequence ...