圖15.14,poly(A)刺激多核糖體(polysome)在mRNA上形成 Stabilityisnotsignificantlydifferent. 空心方塊:空心圓 Translationefficiencyissignificantly different. 圖15.13a 圖15.13b Poly(A)tail對召募polysome到mRNA的影響 Redline: 3 H-labeledpoly(A)-RNA Blueline: 32 P-labeledpoly(A)+RNA Thelonger,thebetter ...
forming a poly(A) tail which can be as long as several hundred nucleotides in the case of nuclear-encoded eukaryotic mRNAs, but only about 50nt for mammalian mitochondrial mRNAs. Polyadenylation is common to all living organisms, but, interestingly, it plays...
Polyadenylation: a tail of two complexes - Proudfoot, O’Sullivan - 2002 () Citation Context ...onucleolytic cleavage followed by addition of a poly(A) tail to the newly generated 3� end (58). This 3� processing is carried out by a complex machinery that associates with Pol II ...
As part of these processing reactions, which take place in the cell nucleus, the 5′ end receives a 7‐methyl guanosine cap , whereas the 3′ end receives a poly(A) tail – a string of adenylate residues not encoded in the genome. These two end modifications are characteristic for all ...
Recent studies have advanced our understanding of the molecular code that dictates the timing of CPEB-mediated poly(A) tail elongation and the extent of translational activation. In addition, evidence for CPEB-independent mechanisms has accumulated, and the breath of biological circumstances in which ...
Poly(A) tail length is controlled by the nuclear poly(A)-binding protein regulating the interaction between poly(A) polymerase and the cleavage and polyadenylation specificity factor. J. Biol. Chem. 284, 22803–22814 (2009). PubMed PubMed Central Google Scholar Eckmann, C. R., Rammelt, ...
-containing reads were extracted from the RNAseq dataset and these reads were used to generate a PAC list. PACs lists generated using the PATseq and RNAseq datasets were compared to assess overlap between the genomic coordinates of PACs in two datasets. As expected very few poly(A) tail-...
The chapter also presents an overview of poly(A) tail labeling and analysis. Concepts related to Ribonuclease digestion and gel electrophoretic analysis are elaborated in depth. The chapter also discusses about polyadenylation assay in vitro. 展开 ...
The polyadenylation process requires two components, the cis-elements on the mRNA and a group of protein factors that recognize the cis-elements and produce the poly(A) tail. Here we report a comprehensive pairwise protein-protein interaction mapping and gene expression profiling of the mRNA ...
The catalytic activity of this protein is responsible for the polymerization of the poly(A) tail. Its function in cleavage is not known. However, one may presume that the ability of poly(A) polymerase to stabilize a CPSF-RNA complex (73, 92) is essential to its role in cleavage. ...