Hall,TM.Poly(A) tail synthesis and regulation: recent structural insights. Current Opinion in Structural Biology . 2002Hall TMT (2002) Poly(A) tail synthesis and regulation: recent structural insights. Current Opinion in Structural Biology 12: 82-88. :...
M.,A Jacobson 摘要: Posttranscriptional maturation of the 3' end of eukaryotic pre-mRNAs occurs as a three-step pathway involving site-specific cleavage, polymerization of a poly(A) tail, and trimming of the newly synthesized tail to its mature length. While most of the factors essential for...
Assembly of a processive messenger RNA polyadenylation complex. Only the complex formed from all three proteins is competent for the processive synthesis of a full-length poly(A) tail.Bienroth... Bienroth,Keller,Wahle - 《Embo Journal》 被引量: 413发表: 1993年 Host factor Hfq of Escherichia ...
②基于二代或三代测序技术表征polyA,已报道的测序方法包括TAIL-seq、PAL-seq、FLAM-Seq和PAIso-Seq等。 2018年诺华公司Beverly M等发表在Anal Bioanal Chem杂志的一篇文章,《Poly A tail length analysis of in vitro transcribed mRNA by LC-MS》,研究基于RNAse T1酶切与oligo dT磁珠纯化得到polyA尾,使用液相色谱...
proportional to the poly(A)-tract length. To account for the density of each cluster, this raw intensity is normalized to that of the fluorescent bases added during sequencing by synthesis 12 , thereby yielding a nor- malized fluorescence intensity for the poly(A) tail of each transcript, pa...
The kit features Poly(A) Polymerase which uses ATP as a substrate for template-independent addition of adenosine monophosphates to the 3′-hydroxyl termini of RNA molecules. The standard protocol was designed to produce a poly(A)-tail length of ~150 b on 60 μg of capped RNA.An alternative...
In contrast, we recently showed that Nab2p regulates polyadenylation during de novo synthesis, and its nuclear location is more consistent with a role in 3'-end processing than that of cytoplasmic Pab1p. Here, we investigate whether PAN activity is required for de novo poly(A) tail synthesis....
Figure 1. Customized Poly(A)-tail Lengths. A 1.4-kb transcript was poly(A)-tailed using various reaction conditions to demonstrate the affect on poly(A)-tail lengths. Each lane contains 0.1 μg of the completed poly(A)-tailing reaction product. ...
We recently developed a poly(A) inclusive full-length RNA isoform-sequencing method (PAIso-seq) at single-oocyte-level sensitivity (a single mammalian oocyte contains ~0.5 ng of total RNA) based on PacBio sequencing that enabled accurate measurement of the poly(A) tail length and non-A ...
摘要: Presents information on a study which determined whether regulation of poly(A) tail length by the poly(A)-limiting element was unique to albumin pre-messenger RNA. Materials and methods of the study; Results and discussion.被引量: 37 ...