3160724Certificate of Analysis2025年1月31日764551KT 3160686Certificate of Analysis2025年1月31日764551KT 2791363Certificate of Analysis2025年1月29日764551KT 显示5 条结果, 在上面搜索特定证书 申请COA 安全数据表 SDS Product Information Long Protocol: USB Poly (A) Tail-Length Assay Kit Protocol订购...
This kit utilizes a novel G/I extension technique which preserves the 3' end of RNAs and ensures accurate measurement of poly(A) tail-length. And, all reaction components have been optimized so you get quality data quickly.
Poly(A) tail (PAT) assayPoly(A)-tail lengthPoly(A)denylomePosttranscriptionalThe poly(A) tail is found on the 3鈥 -end of most eukaryotic mRNAs, and its length significantly contributes to the mRNAs half-life and translational competence. Circadian regulation of poly(A)-tail length is a ...
The procedure described here, the PCR poly(A) test (PAT), allows the analysis of the poly(A) tail on a specific mRNA within a pool of total RNA in a rapid (1 day) and sensitive (subnanogram total RNA) fashion. The assay also provides quantitative estimates of the RNAs poly(A) tail...
Importantly, the use of the ligation-mediated polyA tail assay (LM-PAT) revealed that the PROLIFERA (PRL1) mRNA encoding the MCM7 protein showed almost a doubling in the average length of its poly(A) tail in PARN mutants. Poly(A) tail length in this PARN mutant, however, was no...
Here we report that overexpression of HuD protein in PC12 cells stabilizes the GAP-43 mRNA by delaying the onset of mRNA degradation and that this process depends on the size of the poly(A) tail. Using a polysome-based in vitro mRNA decay assay, we found that addition of recombinant HuD...
Analysis of Poly(A) Tail Lengths by PCR - The PAT Assay 热度: 相关推荐 ARTICLE doi:10.1038/nature13007 Poly(A)-tailprofilingrevealsan embryonicswitchintranslationalcontrol AlexanderO.Subtelny 1,2,3,4 *,StephenW.Eichhorn 1,2,3 *,GraceR.Chen 1,2,3 ,HazelSive 2,3 &DavidP.Bartel 1,2...
In order to independently validate the microarray results, the poly(A) tail lengths of randomly chosen transcripts were directly measured using the ligation-mediated poly(A) tail length (LM-PAT) assay25 as well as Poly(A) Length (PAL) assay26 (Fig. 1e,f). These assays revealed that ...
To study this regulation, we have developed an RNA injection assay that faithfully recapitulates the regulation of the endogenous hb message. Previous studies have suggested that NOS and PUM can regulate translation by directing poly(A) removal. We have found that RNAs that lack a poly(A) tail...
This analysis showed that if the last templated nucleotide of the oma-1 mRNA fragment was an A or a C, then RDE-3 was equally likely to add a U or a G as the first nucleotide of an elongating pUG tail. If, however, the last templated nucleotide was a U or G, then RDE-3 ...