T cellsGENE expressionDATA analysisBoth poly(A) enrichment and ribosomal RNA depletion are commonly used for RNA sequencing. Either has its advantages and disadvantages that may lead to biases in the downstream analyses. To better access these effects, we carried out both ribosomal RNA-depleted and...
RNA sequencing (RNA-seq) that utilises next-generation sequencing (NGS) is a powerful tool to understand transcriptional diversity and regulation at bulk and single-cell level. Using RNA-seq, we not only can perform differential gene expression analysis with better resolution, but also comprehensivel...
clinical RNA sequencing. Our evaluation revealed that a small number of lncRNAs and small RNAs made up a large fraction of the reads in the rRNA depletion RNA sequencing data. Thus, we recommend that these RNAs are specifically depleted to improve the sequencing depth of the remaining RNAs. Int...
Objectives The sequencing by the PolyA selection is the most common approach for library preparation. With limited amount or degraded RNA, alternative protocols such as the NuGEN have been developed. However, it is not yet clear how the different library preparations affect the downstream analyses ...
The goal of NanoTail is to provide a set of functions to manipulate and analyze data coming from polyA lengths estimations done using Oxford Nanopore Direct RNA sequencing and Nanopolish software. Existing solutions, like Pipeline for testing shifts in poly(A) tail lengths estimated by nanopolish ...
2023年,Circulation杂志在线发表了一篇题为《Assessment of the Cardiac Noncoding Transcriptome by Single-Cell RNA Sequencing Identifies FIXER, a Conserved Profibrogenic Long Noncoding RNA》的研究论文。该研究通过单细胞RNA测序对心脏非编码转录组进行全面评估,成功鉴定出一个保守的促纤维化长链非编码RNA,命名为FIX...
但是慢慢的科研热点转到了lncRNA,虽然lncRNA只有部分具有polyA尾结构,但也意味着公共数据库里面海量的mRNA-seq表达矩阵里面,都是可以提取到lncRNA部分,新的分析图表就出来了。在很多综述或者教程都可以看到对lncRNA的这样的总结: 1.长度在200-100,000nt
Deep sequencing analysis of RNAs from a grapevine showing Syrah decline symptoms reveals a multiple virus infection that includes a novel virus In a search for viruses associated with decline symptoms of Syrah grapevines, we have undertaken an analysis of total plant RNA sequences using Life Scienc...
Cui, Peng, Qiang Lin, Feng Ding, Chengqi Xin, Wei Gong, Lingfang Zhang, Jianing Geng et al. "A comparison between ribo-minus RNA-sequencing and polyA-selected RNA-sequencing." Genomics 96, no. 5 (2010): 259-265.Cui, P., Lin, Q., Ding, F., Xin, C., Gong, W., Zhang, L....
Global overview of polyA-minus RNA sequencing in breast cancer tissues and matched adjacent cancer tissues.Xianfeng, DingLimin, ZhuTing, JiXiping, ZhangFengmei, WangShaoju, GanMing, ZhaoHongjian, Yang