RNA sequencingT cellsGENE expressionDATA analysisBoth poly(A) enrichment and ribosomal RNA depletion are commonly used for RNA sequencing. Either has its advantages and disadvantages that may lead to biases in the downstream analyses. To better access these effects, we carried out both ribosomal RNA...
RNA sequencing (RNA-seq) that utilises next-generation sequencing (NGS) is a powerful tool to understand transcriptional diversity and regulation at bulk and single-cell level. Using RNA-seq, we not only can perform differential gene expression analysis with better resolution, but also comprehensivel...
RNA sequencing (RNA-seq) has revolutionized the way biologists examine transcriptomes and has been successfully applied in biological research, drug discovery, and clinical development1,2,3. Compared with microarray-based transcriptome profiling, RNA-seq has a wider dynamic range and avoids some of th...
全长转录组应用系列一:可变polyA检测 全长转录组(Iso-Seq)是指利用三代单分子实时测序技术(SMRT),无需对RNA进行打断和拼接,即可直接获得完整的全长转录本。由于该方法可以获得全长转录本,因此与二代短序列测序技术的RNA-seq对比,侧重于转录本结构的分析,能够准确识别转录本同源异构体(isoform)、可变剪切、可变polyA...
[6] Hwang B, Lee JH, Bang D. Single-cell RNA sequencing technologies and bioinformatics pipelines. Exp Mol Med. 2018 Aug 7;50(8):1-14. doi: 10.1038/s12276-018-0071-8. Erratum in: Exp Mol Med. 2021 May;53(5):1005. doi: 10.1038/s12276-021-00615-w. PMID: 30089861; PMCID: PMC...
都说lncRNA只有部分具有polyA尾结构,请证明 通常大家提到转录组测序,指的是mRNA-seq,在测序文库构建的实验阶段我们有两个选项: 去除rRNA 富集polyA 因为真核生物的mRNA都是有polyA尾巴结构,示意图如下: 但是慢慢的科研热点转到了lncRNA,虽然lncRNA只有部分具有polyA尾结构,但也意味着公共数据库里面海量的mRNA-seq表达...
PCR based assays like LM-PAT and ePAT, next generation sequencing methods such as TAIL-seq and PAL-seq. LC-MC 优缺点比较: *下一代测序方法具有最高的分辨率(TAIL-seq报道为1个碱基) 使用凝胶或色谱法的方法通常会在尾巴长度不同的种群中出现偏差(smears) ...
Using transgenic mice with fluorescently labeled germ cells and fluorescence-activated cell sorting (FACS), we isolated germ cells from Dazl KO testes and WT controls and used RNA-sequencing (RNA-seq) to identify mRNAs that are sensitive to DAZL deletion. Integrating the RNA-seq and DAZL-RNA ...
The goal of NanoTail is to provide a set of functions to manipulate and analyze data coming from polyA lengths estimations done using Oxford Nanopore Direct RNA sequencing and Nanopolish software. Existing solutions, like Pipeline for testing shifts in poly(A) tail lengths estimated by nanopolish ...
Also, the poly-A analysis modules were built based on EST sequencing and are not yet updated for use with next-gen RNA-Seq analysis. Add a custom footer Pages 14 PASA Pipeline Wiki Home Software Installation Instructions Running PASA via Docker Running PASA via Singularity Running the ...