全长转录组应用系列一:可变polyA检测 全长转录组(Iso-Seq)是指利用三代单分子实时测序技术(SMRT),无需对RNA进行打断和拼接,即可直接获得完整的全长转录本。由于该方法可以获得全长转录本,因此与二代短序列测序技术的RNA-seq对比,侧重于转录本结构的分析,能够准确识别转录本同源异构体(isoform)、可变剪切、可变polyA...
全长转录组应用系列一:可变polyA检测 全长转录组(Iso-Seq)是指利用三代单分子实时测序技术(SMRT),无需对RNA进行打断和拼接,即可直接获得完整的全长转录本。由于该方法可以获得全长转录本,因此与二代短序列测序技术的RNA-seq对比,侧重于转录本结构的分析,能够准确识别转录本同源异构体(isoform)、可变剪切、可变polyA...
RNA sequencingT cellsGENE expressionDATA analysisBoth poly(A) enrichment and ribosomal RNA depletion are commonly used for RNA sequencing. Either has its advantages and disadvantages that may lead to biases in the downstream analyses. To better access these effects, we carried out both ribosomal RNA...
RNA sequencing (RNA-seq) has revolutionized the way biologists examine transcriptomes and has been successfully applied in biological research, drug discovery, and clinical development1,2,3. Compared with microarray-based transcriptome profiling, RNA-seq has a wider dynamic range and avoids some of th...
Both poly(A) enrichment and ribosomal RNA depletion are commonly used for RNA sequencing. Either has its advantages and disadvantages that may lead to biases in the downstream analyses. To better access these effects, we carried out both ribosomal RNA-de
Objectives The sequencing by the PolyA selection is the most common approach for library preparation. With limited amount or degraded RNA, alternative protocols such as the NuGEN have been developed. However, it is not yet clear how the different library preparations affect the downstream analyses ...
PCR based assays like LM-PAT and ePAT, next generation sequencing methods such as TAIL-seq and PAL-seq. LC-MC 优缺点比较: *下一代测序方法具有最高的分辨率(TAIL-seq报道为1个碱基) 使用凝胶或色谱法的方法通常会在尾巴长度不同的种群中出现偏差(smears) ...
2023年,Circulation杂志在线发表了一篇题为《Assessment of the Cardiac Noncoding Transcriptome by Single-Cell RNA Sequencing Identifies FIXER, a Conserved Profibrogenic Long Noncoding RNA》的研究论文。该研究通过单细胞RNA测序对心脏非编码转录组进行全面评估,成功鉴定出一个保守的促纤维化长链非编码RNA,命名为FIX...
The goal of NanoTail is to provide a set of functions to manipulate and analyze data coming from polyA lengths estimations done using Oxford Nanopore Direct RNA sequencing and Nanopolish software. Existing solutions, like Pipeline for testing shifts in poly(A) tail lengths estimated by nanopolish ...
Deep sequencing analysis of RNAs from a grapevine showing Syrah decline symptoms reveals a multiple virus infection that includes a novel virus In a search for viruses associated with decline symptoms of Syrah grapevines, we have undertaken an analysis of total plant RNA sequences using Life Scienc...