RNA poly(A) tails were thought to be only composited of A residues. Through TAIL-seq, pervasive 3′ end G and U modifications have been found in RNA poly(A) tails with a vital role in mRNA stability in human cell lines2,3. By using the same method, 3′ end of maternal transcripts ...
Ribonucleotidyl transferases (rNTases) add untemplated ribonucleotides to diverse RNAs. We have developed TRAID-seq, a screening strategy in Saccharomyces cerevisiae to identify sequences added to a reporter RNA at single-nucleotide resolution by overexp
产品类型:RNA-Seq建库 品牌:Lucigen 货号:KL11101K 规格:1 价格:3557 产品介绍 Exo-Minus Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both of the 5′→3′ and 3′→5′ exonuclease activities of E. coli DNA Polymerase I from which it is derived. This N-terminal truncat...
U.S. Pat. No. 5,990,069 discloses a lipase from a strain ofFusarium solanivar.minus. A lipase/phospholipase fromFusarium oxysporumand its sequence are disclosed in WO 98/26057. SUMMARY OF THE INVENTION The inventors have isolated a gene encoding a lipolytic enzyme fromFusarium solaniMUCL ...
We find that integrating a number of computational methods to detect genes with differentially retained introns provides a strategy to enrich for alternatively spliced exons in mammalian RNA-seq data, when complemented by RNA-seq analysis of purified cells with experimentally perturbed RNA-binding ...