图3 在质粒转化培养过程中poly A尾巴缩短，poly A尾巴越长，越不稳定 2016年，Kyle Jacoby等人[2]发表文章peVl: A linear Plasmid for generating mRnA IVt templates With extended encoded Poly(A) sequences，首次研究携带不同长度poly A的质粒在细菌扩增过程中尾巴的长度稳定性情况，他们发现，poly A尾巴长度...
Segmented poly(A) tails significantly reduce recombination of plasmid DNA without affecting mRNA translation efficiency or half-life. A convenient method to generate and maintain poly(A)-encoding DNA sequences required for in vitro transcription of mRNA. peVl: A linear Plasmid for generating mRnA IV...
2016年,Kyle Jacoby等人[2]发表文章peVl: A linear Plasmid for generating mRnA IVt templates With extended encoded Poly(A) sequences,首次研究携带不同长度poly A的质粒在细菌扩增过程中尾巴的长度稳定性情况,他们发现,poly A尾巴长度是72 bp时,转化细菌,过夜培养后,其长度可以保持不变;当poly A尾巴加长到1...
Impact of Promoters and PolyA Sequence on Expression Efficiency of Buffalo Mammary Gland Specific Expressing Vectors启动子PolyA序列促乳素绿色荧光蛋白基因表达水牛为了获得能有效表达促乳素的乳腺特异性表达载体,本研究系统比较了不同长度的启动子和有/无PolyA序列对乳腺特异性表达载体表达标记基因绿色荧光蛋白(GFP)...
关键词: Alu; SV40 PolyA; AATAAA 信号; GFP; 转录终止 The effects of SV40 PolyA sequence and its AATAAA signal on up-stream GFP gene expression and transcription termination LI Shu-Ping 1, 2 , FENG Jing-Jing 3 , WANG Hong-Gang 4 , WANG Xiu-Fang 1 , LV Zhan-Jun 1 1. Department of...
关键词:Alu;SV40PolyA;AATAAA信号;GFP;转录终止TheeffectsofSV40PolyAsequenceanditsAATAAAsignalonup-streamGFPgeneexpressionandtranscriptionterminationLIShu-Ping1,2,FENGJing-Jing3,WANGHong-Gang4,WANGXiu-Fang1,LVZhan-Jun11.DepartmentofGenetics,HebeiKeyLabofLaboratoryAnimal,HebeiMedicalUniversity,Shijiazhuang050017,...
【化】 聚腺苷酸 分类: 化学化工|查看相关文献(pubmed)|免费全文文献 详细解释: 以下为句子列表: 英文: The effects of SV40 PolyA sequence and its AATAAA signal on upstream GFP gene expression and transcription termination 中文: SV40 PolyA序列及其AATAAA信号对上游GFP基因表达及转录终止的影响更详细... ...
[8] Lai WC, Zhu M, Belinite M, Ermolenko DN, et al. Intrinsically Unstructured Sequences in the mRNA 3' UTR Reduce the Ability of Poly(A) Tail to Enhance Translation. J Mol Biol. 2022 Dec 30;434(24):167877. doi: 10.1016/j.jmb.2022.167877. ...
网络多聚腺苷酸共有序列 网络释义 1. 多聚腺苷酸共有序列 ...penreadingframe,ORF),3′端有多聚腺苷酸共有序列(polyAconsensussequence)和一个polyA尾;三个起始外显子1~3位于t(3;… www.yp900.com|基于 1 个网页
PolyA+ Elementdoi:10.1007/978-1-4020-6754-9_13154Transposons without long terminal repeats but poly-A sequences at the 3′-OH end. The RNA elements are usually mobilized via a DNA transcript with the aid of their encoded reverse transcriptase. Such......