GST-pulldown studies revealed that many of the mutants bind weaker to the S5a subunit of the proteasome, compared with wild type (WT) ubiquilin-2 protein. The results suggest the mutant proteins are unable to deliver their captured cargo to the proteasome for degradation, which presumably leads...
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Selective small molecule induced degradation of the BET bromodomain protein BRD4. ACS Chem. Biol. 10, 1770–1777 (2015). Article CAS PubMed PubMed Central Google Scholar Nakasone, M. A. et al. Structure of UBE2K-Ub/E3/polyUb reveals mechanisms of K48-linked Ub chain extension. Nat. ...
We further confirmed the binding of Fbxl7 to ubiquitin after expression in cells with Fbxl7-V5 and HA-ubiquitin (HA-Ub) plasmids under MG132 treatment (Figure 1e). Collectively, these results indicate that Fbxl7 degradation is mediated by the ubiquitin- proteasome system. Fbxl18 targets Fbxl7...
Gram-negative bacteria, Legionella pneumophila, meta-effector protein SidJ, successively employ SidE family ADP ribosyltransferase (ART) and phosphodiesterase (PDE) activities to catalyze the covalent ligation of ubiquitin (Ub) to Ser residues on substrate proteins independent of E1 or E2 Ub-activating...
PARP,a 116 kDa nuclear poly (ADP-ribose) polymerase,is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs...
PolyUb, polyubiquitin chain. for understanding how p47 induces the lysosomal degradation of polyubiquitinated NEMO. DUBs, such as A20 and CYLD, have pivotal roles in the negative regulation of NF-κB activation27–30. CYLD is a tumour suppressor protein whose inactivation results in familial ...
b, Intrinsic lysine reactivity of the UbcH5c∼Ub conjugate with and without iso-ADPr. iso-ADPr does not enhance the reactivity of the conjugate in the absence of RNF146. c, Oriole-stained E2∼Ub/lysine reactivity with increasing iso-ADPr (3 min after lysine addition). The rate of ...
In silico 3D structural analysis of Gag Poly Protein in HIV type 1 by HMM and neural networks 来自 ResearchGate 喜欢 0 阅读量: 17 作者:A Anand,UB Jason 摘要: Protein secondary structure prediction remains an open and important problem in life sciences as a first step towards the crucial ...
Ubiquitin-ribosomal proteinS27a(UBRPS27a) is a fusion protein of Ubiquitin andribosomal protein. 泛肽-核糖体蛋白S27a(Ubiquitin-ribosomal protein S27a,UBRS27a)是泛肽和核糖体蛋白的融合蛋白,N端为泛肽,C端由含C2-C2型锌指结构域的高度保守核糖体蛋白S27a构成。