Continued NAME Source AT-accession Target Function References RB38 Chlamydomonas - - - CRS1 Maize AT5G16180 ...C.B. Yohn, A. Cohen, A. Danon, S.P. Mayfield, A poly(A) binding protein functions in the chloroplast as a message-specific translation factor, Proc. Natl Acad. Sci. U. S...
Eukaryotic mRNA has a cap structure and a poly(A) tail at the 5′ and 3′ ends, respectively. The cap structure is recognized by eIF (eukaryotic translation initiation factor) 4 F, while the poly(A) tail is bound by poly(A)-binding protein (PABP). PAB
Nab2p uses an Arg-Gly-Gly (RGG) domain for binding. This protein also contains a Cys-Cys-Cys-His zinc-binding motif, similar to one in RNA polymerase subunits, and a glutamine-rich region that contains a variable number of Gln-Gln-Gln-Pro...
In contrast, SG are only present in the cytoplasm of stressed cells and differ from P-bodies in protein composition and function22. SG usually contain mRNAs bound with translation initiation factors, 40S ribosomal subunits and the poly(A) binding protein, and are therefore believed to represent ...
We identified the “poly(A)-binding protein-interacting protein 2” (PAIP2) as a new VEGF mRNA 3′-UTR interacting protein. By RNA electromobility shift assays, we showed that PAIP2 binds to two distinct regions of a domain encompassing base 1 to 1280 of the VEGF 3′-UTR. Such in ...
Definition Nuclear poly(A) binding protein 1 (PABPN1) designates a protein that binds to the growing poly(A) tails of mRNA precursors during nuclear processing. The protein is required for efficient poly(A) synthesis, and for limiting the length of the poly(A) tail in a mammalian cell to...
图1 真核细胞质中PABPC结构域细胞质多聚腺苷酸结合蛋白(polyadenylate-binding protein,PABPC)对于poly(A) 尾在介导转录物翻译和稳定性中的作用是必要和充分的。PABPC在真核生物中高度保守,具有四个N端RNA识别基序(RNA recognition motif,RRM)结构域,它们以纳摩尔亲和力结合poly(A) 尾(图1)。RRM和RRM2对poly(A...
研究发现，具有非常短的poly(A)尾巴的mRNA通常不能正常翻译。当mRNA的poly(A)尾巴多腺苷酸化，翻译被激活，这表明mRNA翻译受到poly(A)尾长的影响。图1 真核细胞质中PABPC结构域 细胞质多聚腺苷酸结合蛋白（polyadenylate-binding protein，PABPC）对于poly(A) 尾在介导转录物翻译和稳定性中的作用是必要和充分的...
Our work provides the structure of a multidomain RNA-binding protein recognizing single-stranded RNA. The RRMs are arranged in tandem creating a long, narrow RNA-binding trough. The two antiparallel β sheets serve as the base of the trough, with interstrand loops and the domain linker forming...
Nonsense-mediated mRNA decay (NMD) is a cellular process that eliminates messenger RNA (mRNA) substrates with premature translation termination codons (PTCs). In addition, NMD regulates the expression of a number of physiological mRNAs, for example trans