然而以TAILseq、PALseq、FLAMseq、PATseq及纳米孔测序为代表的测序技术通量大、设备要求高，鉴于体外合成的mRNA通量较小，无法体现测序技术的高通量优势。参考文献：[1] Beverly M, Hagen C, Slack O. Poly A tail length analysis of in vitro transcribed mRNA by LC-MS. Anal Bioanal Chem. 2018 Feb;410(...
②基于二代或三代测序技术表征polyA,已报道的测序方法包括TAIL-seq、PAL-seq、FLAM-Seq和PAIso-Seq等。 2018年诺华公司Beverly M等发表在Anal Bioanal Chem杂志的一篇文章,《Poly A tail length analysis of in vitro transcribed mRNA by LC-MS》,研究基于RNAse T1酶切与oligo dT磁珠纯化得到polyA尾,使用液相色谱...
然而以TAILseq、PALseq、FLAMseq、PATseq及纳米孔测序为代表的测序技术通量大、设备要求高,鉴于体外合成的mRNA通量较小,无法体现测序技术的高通量优势。 参考文献: [1] Beverly M, Hagen C, Slack O. Poly A tail length analysis of in vitro transcribed mRNA by LC-MS. Anal Bioanal Chem. 2018 Feb;410(...
②基于二代或三代测序技术表征polyA,已报道的测序方法包括TAIL-seq、PAL-seq、FLAM-Seq和PAIso-Seq等。 2018年诺华公司Beverly M等发表在Anal Bioanal Chem杂志的一篇文章,《Poly A tail length analysis of in vitro transcribed mRNA by LC-MS》,研究基于RNAse T1酶切与oligo dT磁珠纯化得到polyA尾,使用液相色谱...
2018年诺华公司Beverly M等发表在Anal Bioanal Chem杂志的一篇文章,《Poly A tail length analysis of in vitro transcribed mRNA by LC-MS》,研究基于RNAse T1酶切与oligo dT磁珠纯化得到polyA尾,使用液相色谱质谱联用(LC-MS)检测polyA尾长度的分布,目前该策略是当前mRNA体外制备工艺中较为常用的方法。
②基于二代或三代测序技术表征polyA,已报道的测序方法包括TAIL-seq、PAL-seq、FLAM-Seq和PAIso-Seq等。 2018年诺华公司Beverly M等发表在Anal Bioanal Chem杂志的一篇文章,《Poly A tail length analysis of in vitro transcribed mRNA by LC-MS》,研究基于RNAse T1酶切与oligo dT磁珠纯化得到polyA尾,使用液相色谱...
2018年诺华公司Beverly M等发表在Anal Bioanal Chem杂志的一篇文章,《Poly A tail length analysis of in vitro transcribed mRNA by LC-MS》,研究基于RNAse T1酶切与oligo dT磁珠纯化得到polyA尾,使用液相色谱质谱联用(LC-MS)检测polyA尾长度的分布,目前该策略是当前mRNA体外制备工艺中较为常用的方法。
2018年诺华公司Beverly M等发表在Anal Bioanal Chem杂志的一篇文章,《Poly A tail length analysis of in vitro transcribed mRNA by LC-MS》,研究基于RNAse T1酶切与oligo dT磁珠纯化得到polyA尾,使用液相色谱质谱联用(LC-MS)检测polyA尾长度的分布,目前该策略是当前mRNA体外制备工艺中较为常用的方法。
Poly A tail length analysis of in vitro transcribed mRNA by LC-MSmRNAPoly ATail lengthMass spectrometrySlippageIn vitro transcriptionID="Abs1" Language="En" OutputMMichaelNovartisBeverlyNovartisCaitlinNovartisHagenNovartisOlgaNovartisSlackNovartisANALYTICAL AND BIOANALYTICAL CHEMISTRY...
The procedure described here, the PCR poly(A) test (PAT), allows the analysis of the poly(A) tail on a specific mRNA within a pool of total RNA in a rapid (1 day) and sensitive (subnanogram total RNA) fashion. The assay also provides quantitative estimates of the RNAs poly(A) tail...