we show that poly(A) tail length and internal non-A residues are highly dynamic during the human OET, using poly(A)-inclusive RNA isoform sequencing (PAIso-seq). Unexpectedly, maternal
Fig. 1: RNA poly(A) tail length anticorrelates with RNA expression. a Schematic diagram of the DRS technique used to estimate poly(A) tail lengths. The poly(A)+ fraction is isolated from total RNA; then libraries are prepared according to the protocol, with the first step of (dT)10 ad...
Dorado also supports additional features that can be customized through a configuration file (described below): Custom primer sequence for cDNA tail estimation Clustering of interrupted Poly(A/T) tails Estimation of Poly(A/T) length in plasmids Poly(A/T) Reference Diagram cDNA 5' --- ADAPTER...
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(A) Diagram of the full-length PV1 RNA which encodes all of the viral proteins. (B) Diagram of PV1p50 RNA in which nucleotides 867–6011 from PV1 RNA are deleted. This RNA contains the authentic 5′ NTR, IRES, 3′ NTR and poly(A) tail of PV1 RNA. It also contains the ...
The poly(A) tail is added to pre-mRNA in the nucleus by cleavage and polyadenylation, a process catalyzed by a large complex of proteins. In vertebrates, 3′ end formation depends on two cis-acting RNA elements, the highly conserved AAUAAA sequence found upstream of the polyadenylation site ...
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Figure 10a shows a conceptual energy diagram that explains the photocurrent shown in Fig. 9. The unusual situation Q1 < Q2 is considered in Fig. 10a. The upper part of Fig. 10a shows the situation where PHIG is irradiated with white light, and the state where optical carriers are gen...
Fig. 1: High-resolution cryo-EM structure of a CRL promoting poly-ubiquitylation. a, Schematic representation for all protein subunits used to form the chain formation complex for cryo-EM. b, Diagram comparing the native transition-state geometry for UBE2R2-dependent chaining with a stabilized ub...