Understanding the function of poly(A) tails has been challenging because of difficulties in measuring their lengths in a genome-wide manner. Bartel and colleagues now report a new high-throughput sequencing method, called PAL-seq ('poly(A)-tail profiling by sequencing'), that accurately measures...
Poly(A) tails enhance the stability and translation of most eukaryotic mRNAs, but difficulties in globally measuring poly(A)-tail lengths have impeded greater understanding of poly(A)-tail function. Here, we describe poly(A)-tail length profiling by sequencing (PAL-seq) and apply it to measur...
suringpoly(A)-taillengthshaveimpededgreaterunderstandingofpoly(A)-tailfunction.Herewedescribepoly(A)-tail lengthprofilingbysequencing(PAL-seq)andapplyittomeasuretaillengthsofmillionsofindividualRNAsisolatedfrom yeasts,celllines,Arabidopsisthalianaleaves,mouseliver,andzebrafishandfrogembryos.Poly(A)-taillengthswere...
通过eIF4E-eIF4G-PABPC在mRNA “闭环”中5'和3'末端之间的物理相互作用为poly(A)尾如何影响脱帽提供了可能的解释。这些相互作用稳定了cap上的eIF4E[5],并可以防止脱帽酶的结合。因此,poly(A)尾可能通过PABPC抑制帽和脱帽酶之间的结合来维持mRNA的稳定性。 Poly(A)尾长影响翻译效率 图4 poly(A)尾长影响翻译...
Global investigation of poly(A) tails has been hindered by technical challenges. In a recent advance, two groups developed deep sequencing methods to globally interrogate poly(A) tail length and sequence with high precision, opening new avenues for investigation of poly(A) tail functions in mRNA ...
Poly(A)尾促进mRNA翻译 Poly(A)尾有助于mRNA的翻译状态和稳定性,作为细胞质中基因表达的主要调节因子发挥作用。具体来说,poly(A)尾可以与mRNA 5'端上的7-甲基鸟苷(7-methylguanosine,m7 G)帽协同作用以刺激翻译。哺乳动物的poly(A)尾平均长度约为200nt,其在酵母中平均约70nt。研究发现,具有非常短的poly(A...
poly(A) tailpolyadenylationThe prevention and treatment efficiency of ethanol-induced gastric epithelial injury are not satisfied. We have previously shown that AMP-activated protein kinase (AMPK) activation exerts a pro-survival function in human gastric epithelial cells (GECs). miroRNA-451 ("miR-...
期刊的一篇综述,《Measuring the tail: Methods for poly(A) tail profiling》,总结了利用二代(Illumina)或三代(PacBio)测序与RNA直接测序平台检测polyA尾,可在分析polyA长度分布的同时表征序列的完整性和准确性。 一、基于内切酶与LC-MS平台的polyA尾检测 ...
在RNA世界中，A核苷酸似乎在提供有益特性方面更加突出。它与其他核苷酸相比，更容易稳定mRNA分子的结构，...
J Lim,M Lee,A Son,H Chang,VN Kim 摘要: Eukaryotic mRNAs are subject to multiple types of tailing that critically influence mRNA stability and translatability. To investigate RNA tails at the genomic scale, we previously developed TAIL-seq, but its low sensitivity precluded its application to ...