Message RNA poly(A) tails are vital for their function and regulation. However, the full-length sequence of mRNA isoforms with their poly(A) tails remains undetermined. Here, we develop a method at single-cell level sensitivity that enables quantification of poly(A) tails along with the full-...
Milcarek C, Price R, Penman S: The metabolism of a poly(A) minus mRNA fraction in HeLa cells. Cell. 1974, 3: 1-10. 10.1016/0092-8674(74)90030-0. Article PubMed CAS Google Scholar Salditt-Georgieff M, Harpold MM, Wilson MC, Darnell JE: Large heterogeneous nuclear ribonucleic acid...
Ribonucleotidyl transferases (rNTases) add untemplated ribonucleotides to diverse RNAs. We have developed TRAID-seq, a screening strategy in Saccharomyces cerevisiae to identify sequences added to a reporter RNA at single-nucleotide resolution by overexp
产品类型:RNA-Seq建库 品牌:Lucigen 货号:KL11101K 规格:1 价格:3557 产品介绍 Exo-Minus Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both of the 5′→3′ and 3′→5′ exonuclease activities of E. coli DNA Polymerase I from which it is derived. This N-terminal truncat...
j Heatmap showing the relative expression of representative genes (down-regulated in Ythdf2CKO) in activated Ythdf2F/F and Ythdf2CKO CD8 T cells from RNA-seq data. Enhanced chromatin accessibility or putative IKZF1/3 binding is depicted by a filled circle. Error bars, mean ± s.e.m....
RNA transcripts processed in alternative ways, which largely failed to be detected by oligo(dT) purification. Here, we describe an enrichment strategy to purify non-polyadenylated (poly(A)−/ribo−) RNAs from total RNAs by removal of poly(A)+ RNA transcripts and ribosomal RNAs. In the ...
To identify the tumor-altered co-expression partners of each TTLL glutamylase, we subtracted the normalized enrichment scores (NES) of enriched (FDR < 0.05) gene sets in tumor signatures minus the solid tissue normal signatures. Differential expression analysis of TTLL11 upon overexpression of ...
the bottom hinge shows the 25% quantile, the top hinge shows the 75% quantile, the bottom whisker shows the smallest observation greater than or equal to bottom hinge minus 1.5 × interquartile range (IQR), and the top whisker shows the largest observation less than or equal to the to...
Such portions may vary in size from the complete amino acid sequence minus one amino acid to peptides of at least 2, preferably at least 5 amino acids. In case the active part of the protein lies in two or more portions of the complete amino acid sequence, the invention also relates to...
The term “about” means plus or minus 0.1 to 10%, 1-10%, or preferably 1-5%, of the number to which reference is being made. Further, the definitions and embodiments described in particular sections are intended to be applicable to other embodiments herein described for which they are ...