The invention discloses a quantitative RT-PCR primer and probe and agent box of PML-RARa fusing gene mRNA fluorescence with BCR-ABL fusing gene primer and probe sequence as SEQ ID NO1-4 and internal reference gene primer and probe sequence as SEQ ID NO5-7, wherein the agent box contains...
法RT—PCR检测PML—RARa融合基因,并作灵敏度试验.结果:本 方法的灵敏度可达到1:103水平.对25例 APL患者检测PML—RARa融合基因,阳性率为100%,并可检测出 PML—RARa融合基因各种融合方式.结论: 一 步法RT—PCR检测PML—RARa融合基因有很好的灵敏度和特异 性,操作简便,快速,特别适用于临床检测. 关键词:急性早幼...
13、 Key words realtime quantitative RTPCR; acute promyelocytic leukemia; PMLRARa fusion gene; minimal residual disease 实时定量RTPCR检测急性早幼粒细胞白血病PMLRARa融合基因 急性早幼粒细胞白血病(APL)具有特异性的染色体易位t(15;17)易位,使17号染色体上的RARa基因与15号染色体上PML基因融合 14、,产生融合...
PML/RARa阳性模板进行扩增,检测51例初诊APL患者PML/RARot转录本含 量,并对检测结果进行凝胶电泳和测序;设计不同异构体的特异性引物,建立异 构体特异性RQ—PCR方法对6种特异性片构体PML/RARa阳性模板进行扩增, 评价异构体定量检测的特异性,并检测45例初诊APL患者中不同异构体的含量。
Key words realtime quantitative RTPCR; acute promyelocytic leukemia; PMLRARa fusion gene; minimal residual disease 实时定量RTPCR检测急性早幼粒细胞白血病PMLRARa融合基因急性早幼粒细胞白血病(APL)具有特异性的染色体易位t(15;17)易位,使17号染色体上的RARa基因与15号染色体上PML基因融合,产生融合基因PMLRARa。实...
We analysed the kinetics of promyelocytic leukaemia–retinoic acid receptor-α (PML–RARα) transcripts by real-time quantitative PCR (RQ-PCR) in bone marrow samples from 184 patients and assessed the prognostic impact of fms-related tyrosine kinase 3–internal tandem duplication (FLT3–ITD) in ...
RT-PCR技术是检测成人急性白血病PML/RARA、MLL、AML1/ETO基因重排的可靠方法,适用于AL的诊断、疗效判定及微小残留病灶的检测。急性早幼粒白血病(APL)是一种时常伴发严重出血的急性白血病.PML/RARA融合基因是APL 的标志性基因,在APL早期诊断和预后监测中具有重要意义.目前PML/RARA融合基因检测的方法主要有染色体分析、...
we performed quantitative real-time RT-polymerase chain reaction (qRT-PCR) analysis to compareCDKN2Dexpression in the presence or absence of PML/RARαin PR9 cells, a PML/RARα-inducible cell line. As shown inFigure 1b,CDKN2Dexpression was markedly decreased after the PML/RARαinduction. These...
2.3. Quantitative real-time PCR (qPCR) qPCR was performed as described [12]. The following primers were used: PML-RARA (forward, CAGTCTCAGCCTTCTCCATCA; reverse, GCTTGTAGATGCGGGGTAGA) [15], PCGF2 (forward, TGACGTGCAGGTCCATAAAA; reverse, ATCACTCAGAGCCCCCTTCT), and GAPDH (forward, TG...
Semiquantitative RT-PCR for retinoid-regulated genes or CD34, CD11b, and lactoferrin was performed using RNA samples made from 3 independent cultures using standard methods.17 In addition, RT-PCR for 18S was used as an internal control to ensure equal loading of samples. Real-time RT-PCR was...