The plus and minus method was an early version of DNA sequencing using dideoxy analogs of nucleosides (+ batch) during replication. After the analog was incorporated to a site, T4 exonuclease failed...doi:10.1007/978-1-4020-6754-9_13051George P. Rédei...
Autonomous parvoviruses are thought to uniquely encapsidate single-stranded DNA of minus polarity. In contrast, the defective adeno-associated viruses separately encapsidate equal amounts of plus and minus DNA strands. We reexamined the uniqueness of minus strand encapsidation for the autonomous parvo...
100bp Plus DNA Ladder Marker为已含有1×loading Buffer的DNA溶液,可取5μl直接电泳,使用方便。100bp Plus DNA Ladder Marker由DNA片段5000bp、3000bp、2000bp、1500bp、1000bp、900bp、800bp、7 00bp、600bp、500bp、400bp、300bp、200bp以及100bp构成,其中500bp的DNA量为100ng,其余条带DNA量约为50ng。
Long terminal repeat elements and retroviruses require primers for initiation of minus and plus-strand DNA synthesis by reverse transcriptase. Here we demo... T.,Heyman,and,... - 《Journal of Molecular Biology》 被引量: 111发表: 1995年 [A detection method of recombinant DNA from genetically...
the minus-strand DNA to the 3' end, where further elongation can lead to production of RC DNA. In duck hepatitis B virus (DHBV), a small terminal redundancy (5'r and 3'r) on the ends of the minus-strand DNA has been shown to be important, but not sufficient, for circularization. ...
All constructs created using PCR were verified by sequencing. Plant growth conditions Plant constructs were transformed into the spr1–6 mutant and an RFP-TUB6 microtubule marker line using an Agrobacterium-mediated floral dip method. To check the localization of SPR1-GFP in the eb1a;eb1b double...
strand DNA template, novel sequences were copied into plus-strand DNA. The generation of these novel sequences could be explained by complete copying of the redundancy at the 5' end of the minus-strand DNA template followed by a template switch and then extension from a mismatched 3' ...
1 A). RT initiates minus strand DNA synthesis by elongation of a specific cellular tRNA primer annealed to the primer binding site (PBS) and continues as far as the 5′ end of the RNA genome, generating the so-called strong stop cDNA (ss-cDNA). The concomitant degradation of the RNA ...
At least 70% of plus or minus strands of adenovirus-associated virus DNA contain self-complementary sequences at or near their termini. Self-annealing of these sequences generates circular molecules that are closed by duplex, hydrogen-bonded segments. The self-annealed segments are sensitive to exo...
Many retroviruses either encode dUTP pyrophosphatase (dUTPase) or package host-derived uracil DNA glycosylase as a means to limit the accumulation of uracil in DNA strands, suggesting that uracil is detrimental to one or more steps in the viral life cycle. In the present study, the effects of ...