A new method for sequencing DNA - M, GILBERT - 1977Maxam, A.M., Gilbert, W. (1977) A new method for sequencing DNA. Proceedings of the National Academy of Sciences of the USA 74: pp. 560Maxam A M,Gilbert W. A new method for sequencing DNA[J].Proceedings of the National Academy ...
In this method, a fragment of DNA carrying the origin of replication from a single-stranded phage, F1 or M13, is inserted into a plasmid to make a single-stranded:double-stranded bifunctional plasmid of the shortened fragments into sequencing vectors. The new family of bifunctional plasmid ...
DNA sequencing is the gold standard method for accurate microbial and viral typing. However, DNA sequencing techniques have been facing limitations in typi... Baback Gharizadeh*, Maria Oggionni, Biying Zheng, Edit Akom, Nader Pourmand*, Afshin Ahmadian, Keng-Ling Wallin|| and Pl Nyrén - 《...
DNA sequencing techniques have evolved rapidly in the last 5 years by the introduction of new sequencing machines, denominated second-generation sequencers, next-generation sequencers or massive parallel sequencers. These technologies make it possible to determine the complete sequence of the human genom...
“The novelty in our work is that we didn’t use sequencing methods widely used in this field, instead we used mass spectrometry, which is the first time this approach has been used to measure DNA modifications on purified, replicated DNA,” says Dr. Stewart-Morgan, co-first author of the...
Single-cell sequencing enables us to better understand genetic diseases, such as cancer or autoimmune disorders, which are often affected by changes in rare cells. Currently, no existing software is aimed at identifying single nucleotide variations or mi
Here, we present a deep learning model (DLM) for predicting Next-Generation Sequencing (NGS) depth from DNA probe sequences. Our DLM includes a bidirectional recurrent neural network that takes as input both DNA nucleotide identities as well as the calculated probability of the nucleotide being ...
"[scPipe: A flexible R/Bioconductor preprocessing pipeline for single-cell RNA-sequencing data" https://doi.org/10.1371/journal.pcbi.1006361 PLOS Computational Biology, 2018. SEQC - Single-Cell Sequencing Quality Control and Processing Software, a general purpose method to build a count matrix ...
In DNA, the loss of a nucleobase by hydrolysis generates an abasic site. Formed as a result of DNA damage, as well as a key intermediate during the base excision repair pathway, abasic sites are frequent DNA lesions that can lead to mutations and strand
1.A method for constructing a sequencing library based on a single-stranded DNA molecule, comprising:(1) forming a poly(C)ntail at a 3′-terminus of the single-stranded DNA molecule, to obtain a single-stranded DNA molecule with the poly(C)ntail with n representing a number of base C...