Here we describe an in vitro plate-based binding assay for characterization of the Sema3/Nrp interaction. This assay utilizes Nrp-affinity plates and an alkaline phosphatase (AP)-Sema3 fusion to rapidly measure direct Sema3/Nrp binding. This assay can be used to measure receptor-ligand binding...
Microtiter plate-based antibody-competition assay to determine binding affinities and plasma/blood stability of CXCR4 ligandsC-X-C chemokine receptor type 4 (CXCR4) is involved in several intractable disease processes, including HIV infection, cancer cell metastasis, leukemia cell progression, rheumatoid...
2.6. PEI and plasmid binding assay The ratio of plasmids to PEI is denoted as N/P, which represents the nitrogen to phosphorus ratio. When the N/P ratio is calculated, nitrogen (N) is sourced from the repeat unit of PEI (NHCH2-CH2) with a molecular weight of 43, whereas phosphorus ...
This protocol describes a fast and robust method for single-cell chromatin accessibility profiling based on the assay for transposase-accessible chromatin using sequencing (ATAC-seq). The method combines up-front bulk Tn5 tagging of chromatin with flow cytometry to isolate single nuclei or cells. ...
activity assay: suitable (protein kinase/phosphatase)bead-based assay: suitableligand binding assay: suitablereceptor binding assay: suitablesolubility assay: suitable H 14.4 mm L 128 mm Show More Related Categories MultiScreen® Filter Plates ...
High Throughput Fliter plate-based melanin binding assayHaspel, HBowman, KMitchell, SWalton, PLiao, DSheldon, A
Development and preliminary validation of a microtiter plate-based receptor binding assay for paralytic shellfish poisoning toxins. Toxicon. 1997; 35 :625–636. [ PubMed ]Doucette, G.; Logan, M.; Ramsdell, J.; Van Dolah, F. Development and preliminary validation of a microtiter plate-based ...
AIMS: To develop a DNA based plate hybridisation assay for the detection of polymerase chain reaction (PCR) products amplified from Aspergillus fumigatus DNA; and to determine the sensitivity of this technique and compare it with Southern blotting. METHODS: A half-log dilution series of DNA ...
The ability of FH-binding protein A (FhbA) to bind FH-like protein 1 (FHL-1) has not been assessed previously. In this study, using a whole-cell absorption assay, we demonstrated that B. hermsii absorbs both FH and FHL-1 from human serum. Consistent with this, affinity ligand bindi...
1990. An ELISA plate- based assay for hyaluronan using biotinylated proteoglycan G1 domain (HA-binding region). Matrix. 10:306-313.Fosang AJ, Hey NJ, Carney SL, Hardingham TE. An ELISA plate-based assay for hyaluronan using biotinylated proteoglycan G1 domain (HA-binding region). Matrix ...