Here we describe an in vitro plate-based binding assay for characterization of the Sema3/Nrp interaction. This assay utilizes Nrp-affinity plates and an alkaline phosphatase (AP)-Sema3 fusion to rapidly measure direct Sema3/Nrp binding. This assay can be used to measure receptor-ligand binding...
4. In contrast to sulphated GAGs, the hyaluronan concentration was highest in synovial fluid (3.53 mg g-1; biotinylated G1 domain binding assay). The... FM Price,JR Levick,RM Mason - 《Journal of Physiology》 被引量: 258发表: 1996年 Spatial and temporal changes in distribution of proteo...
An Elisa Plate Based Assay for Hyaluronan Using Biotinylated Proteoglycan G1 Domain (HA-Binding Region) Hey, NJ, Carney, SL, Hardingham, TE (1990) An ELISA plate based assay for hyaluronan using biotinylated proteoglycan G1 domain (HA-binding region). Ma... AJ Fosang,NJ Hey,SL Carney,.....
Microtiter plate-based antibody-competition assay to determine binding affinities and plasma/blood stability of CXCR4 ligandsC-X-C chemokine receptor type 4 (CXCR4) is involved in several intractable disease processes, including HIV infection, cancer cell metastasis, leukemia cell progression, rheumatoid...
This protocol describes a fast and robust method for single-cell chromatin accessibility profiling based on the assay for transposase-accessible chromatin using sequencing (ATAC-seq). The method combines up-front bulk Tn5 tagging of chromatin with flow cytometry to isolate single nuclei or cells. ...
Carbohydrate-binding modul... 被引量: 0发表: 0年 Lipoprotein lipase enhances the interaction of low density lipoproteins with artery-derived extracellular matrix proteoglycans )PG and dermatan sulfate (DS)PG, the major extracellular PG of the artery wall, a microtiter plate assay was used to ...
High Throughput Fliter plate-based melanin binding assayHaspel, HBowman, KMitchell, SWalton, PLiao, DSheldon, A
Receptor binding assayHuman norovirus is a leading cause of gastroenteritis worldwide. Although two in vitro cultivation methods have been reported, they cannot provide mechanistic insights into viral inactivation. Receptor-binding assays supplement these assays and give insight into capsid integrity. We ...
Therefore, the results were consistent with the binding specificity in a plate-based biochemical assay, as determined using luminescent QDs as a labeling agent.doi:10.1016/j.snb.2004.11.051Chin-Ping HuangDepartment of Applied ChemistryHong-Wei Liu...
We demonstrate that this antibody-competition assay allows a convenient and cheap determination of binding affinities of various CXCR4 antagonists in living cells within just 3 h. Moreover, the assay can be performed in the presence of high concentrations of physiologically relevant body fluids, and...