proved the expression of influenza A virus NP protein.Conclusion The experiment is a success in the construction of eukaryotic expressing plasmids for NP gene,thus providing a basis for further probing into the mechanism of virus infection and physical and chemical factors which can affect the gene...
Construction of Magnetic Small Interfering RNA Nanoparticles and Effects of Its Transfection on Silencing Survivin Gene Expression and Inducing Human Bladd... [Objective] To investigate the construction of the magnetic small interfering RNA (siRNA) plasmid nanoparticles and the effects of its transfection...
Centromeric Plasmids and Artificial Chromosomes: New Kids on the Plasmodium Transfection Blockantigenic variationchromosomeslife cycleplasmidstelomeresPlasmodiumIn this issue of Cell Host & Microbe, Iwanaga and colleagues ( Iwanaga et聽al., 2010) report on the construction of plasmids and artificial ...
Thus, transformation methods exist, for example, for Pseudomonas and related gram-negative bacteria (A. A. Mercer and J. S. Loutit, 1979, Transformation and Transfection of Pseudomonas aeruginosa: Effects of Metal Ions, J. Bacteriol. 140 : 37-42; A. M. Chakrabarty, J. R. Mylroie, D. ...
A DNA transfection system for generation of influenza A virus from eight plasmids. Presents information on a study which developed an eight-plasmid DNA transfection system for the rescue of infectious influenza A virus from cloned cyclic ... Hoffmann,Erich,Neumann,... - 《Proceedings of the ...
To increase the transfection efficacy of the complexed suicide nanoplasmid vectors, a transfection enhancer that we have recently identified in the organism Agrostemma githago L. was applied as well (Clochard et al., 2020). 2. Materials and methods 2.1. Construction of suicide nanoplasmids ...
Controls included an empty vector-immu- nized group and a group that received 1 g of M/M2 protein in combination with a non-expressing plasmid. This group was included to account for the possibility that some M/M2 protein produced during transfection might be contaminating the HPV particle ...
目的 构建PDK1基因短发夹RNA(Short hairpin RNA, ShRNA)慢病毒载体(Lentiviral vector, LV),鉴定其对PC-3人前列腺癌细胞PDK1基因的干扰效应。方法 GeneBank检索PDK1基因序列,参考该序列设计PDK1特异性过干扰序列,分别酶切过表达用载体LV3,纯化酶切产物后进行定向连接。将重组干扰病毒质粒与慢病毒包装质粒共转染293T细...
Transfection and expression of the recombinant plasmid of human IL-10cDNA in cells and rats; 人白细胞介素10 cDNA重组质粒在细胞和大鼠体内的转染和表达 3. Efficient construction and identification of the recombinant plasmid carrying genes coding to Schstosoma japonicum antigen epitope peptides; 日本...
Lentiviral supernatant was collected 24 h and 48 h after transfection, centrifuged and filtered through a 0.45 μm pore size filter. The lentiviral supernatants were directly used for transduction or virus concentration was performed by centrifugation for 4 h at 10,000g through 10% (w/v)...