Thermo Scientific KingFisher Plasmid Pro Prep sizes availableMiniMaxi Purity grade/Endotoxin levelTransfection grade Transfection grade Kits for transfection-grade plasmid DNA purification Transfection-grade plasmid isolation kits are specifically designed for scientists who need to extract plasmid DNA (pDNA) ...
The Thermo Fisher Scientific GeneJET Plasmid Maxiprep Kit allows for efficient isolation of high-quality plasmid DNA from bacterial cultures, recovering up to 750 μg per prep. With a fast 60-minute procedure and a convenient spin column format, this kit offers high yields and con...
PROTOCOL 4: Using the Thermo Scientific S100-AT4 Fixed Angle Rotor Isolation of Plasmid DNA Using the S100-AT4 Fixed Angle Rotor The S100-AT4 rotor is designed to accommodate 3.5 mL polyallomer tubes (PN 75000509), which are ideally suited for density gradient separations. A major advantage...
Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. The kit recovers up to 20 µg of high copy plasmid DNA per isolation procedure.Highlights• Efficient—high yields of up to 20 µg of high quality...
We used the counter-selectableE. coliβ3914/pOXA-48_K8 donor to conjugate plasmid pOXA-48_K8 in the 50 wild-type strains. We used the same protocol described above, but the final conjugation mix was plated on LB with no DAP (to counter-select the donor) and with amoxicillin-clavulanic ...
(Thermo Fisher Scientific). Incorrect patterns are marked with a ‘×’. (E) Electrophoresis on a 0.5% agarose gel of a 50 kb plasmid. Lane 1: GeneRuler high range DNA ladder (Thermo Fisher Scientific). Lane 2: 50 kb plasmid before introducing mutations. Lanes 2–7: 50 kb ...
PRODUCTINFORMATION ThermoScientific GeneJETPlasmidMiniprepKit #K0502,#K0503 .thermoscientific/fermentas #K___ Lot___ CERTIFICATEOFANALYSIS ThermoScientificGeneJETPlasmidMiniprepKithasbeentestedintheisolationofpUC19DNA fromE.coliaccordingtothePURIFICATIONPROTOCOLdescribedonp.p.4-5. ThequalityofisolatedDNAhasbeen...
ESBL-pAmpC-producing ETEC isolates were subjected to partial sequencing using the Big Dye Terminator V3.1 sequencing kit (Applied Biosystems, ThermoFisher Scientific, USA) in an ABI 3500 Genetic Analyzer (Applied Biosystems, ThermoFisher Scientific, USA) according to the manufacturer's protocol. The...
TheThermo Fisher GeneJET Plasmid Miniprep Kitis a spin column-based kit that utilizes silica-based membrane technology. It yields high-quality plasmid DNA, recovering up to 20 μg per isolation procedure. With a fast and convenient procedure, this kit eliminates ...
The PureLink HiPure Plasmid Filter Purification Kits allow isolation of high yields of highly pure plasmid DNA. The HiPure Filter Column enables rapid clearing of the bacterial lysate without the need for centrifugation. The lysate Filtration Car...