Using our advanced-transfection grade plasmid isolation kits can help eliminate the risks associated with endotoxins and obtain pure plasmid DNA for your scientific endeavors. Thermo Scientific GeneJET Endo-Free Plasmid Maxiprep KitInvitrogen PureLink Expi Endotoxin-Free Plasmid Purification Kits Prep sizes...
Plasmid DNA Isolation DNA Learning CenterPlasmid kit selection tool Thermo Fisher Scientific offers a wide range of plasmid DNA extraction products that are designed to isolate plasmid DNA at the purity and scale you need. Choose from our selection of high-performing, cost-effective p...
Plasmid Isolation Kit 货号 60600K-100T 规格 100T 运输条件 常温 储存条件 按标签提示 产品内容 Plasmid spin Columns 2 mL Collection Tubes Buffer P1 Buffer P2 Buffer P3 Buffer PD Buffer PW (使用前添加60 mL 无水乙醇) Elution Buffer RNase A(100mg/mL) ...
all wash steps in the Monarch Plasmid DNA Miniprep Kit protocol are required. All steps should be performed as described to ensure the recovery of high-quality plasmid DNA.
This Protocol is designed to isolate 500-1200 ug of high Copy-Number plasmids or 50-400 ug of low Copy-Number Plasmids from 200 ml overnight cultures. 实验步骤 Growth of bacterial culture: 1. Culture volume: Inoculate 200 ml LB/ampicillin (50 ug/ml) medium placed in a 1-4 liter cultur...
The ISOLATE II Plasmid Mini Kit provides a simple, efficient column-based method for the isolation of plasmid DNA from bacterial cultures, without the need for hazardous reagents such as phenol. By combining SDS/alkaline lysis with the speed and ease-of-use of silica-membrane purification, the ...
Are plasmids recovered using the Monarch Plasmid Miniprep Kit endotoxin free? I have used 5 ml of cell culture for plasmid isolation with the Monarch Plasmid Miniprep kit and I am obtaining low amounts of plasmid and/or contaminating gDNA. Why is this, and what are your suggestions to impro...
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上海古朵生物供应PlasmidMAX™ DNA Isolation Kit 50 Preps使用说明书,上海古朵生物是一家集研发、销售为一体的技术生物企业,公司专注于生命科学和生物技术领域,专业提供分子生物学、免疫学、生命科学基础研究以及临床检测等诸多领域的试剂、耗材、仪器等各类产品及生物技术服务。 Epicentre生物技术公司是一家核酸样品制备...
were transferred into new strains either by electroporation or by conjugation using a biparental mating method. Plasmids were isolated by a modified alkaline lysis protocol as described in Sambrook and Russell (2001)56or by using the Qiagen®Plasmid Midi Isolation Kit (Qiagen, Valencia, CA, USA)...