There are hundreds of protocols published for isolation and purification of plasmid DNA and still the search is on for the cost and time saving methods. Each protocol published has its own advantages and limitation and the plasmid DNA obtained by different protocols vary in purity and yield. ...
About this protocol Cite this protocol Ehrt, S., Schnappinger, D. (2003). Isolation of Plasmids fromE. coliby Alkaline Lysis. In: Casali, N., Preston, A. (eds)E. coliPlasmid Vectors. Methods in Molecular Biology™, vol 235. Humana Press. https://doi.org/10.1385/1-59259-409-3:75...
The characterization of recombinant mycobacterial genes has routinely been time-consuming and difficult because of the laborious procedures required for isolating plasmids from mycobacteria. We developed a rapid protocol for isolating plasmid DNA frommycobacteria using a modification of a mini-plasmid purifica...
Choose from our selection of high-performing, cost-effective plasmid purification technologies designed to help you overcome common plasmid prep issues, such as low recovery and the presence of impurities. Learn how to manually purify plasmid DNA using our kits and our plasmid isolation ...
Choose from our selection of high-performing, cost-effective plasmid purification technologies designed to help you overcome common plasmid prep issues, such as low recovery and the presence of impurities. Learn how to manually purify plasmid DNA using our kits and our plasmid isolation protocol ...
I have developed a protocol that combines these two steps by selecting serially diluted bacteria in liquid cultures grown in 48-well tissue-culture dishes. Here I describe howthis procedure makes it possible to complete the entire process from ligation to the isolation of recombinant plasmid DNA ...
NucleoBond PC 100 (20) 20 preps for the isolation of plasmid DNA Buffer S1, S2, S3, N2, N3, N5 RNase A NucleoBond AX 100 columns, plastic washers, NucleoBond folded filters Protocol中文名: 中文别名: CBNumber: CB1978939 分子式: 分子...
blaIMP-4. Thus far, In this study we report the first isolation of carbapenemase (IMP-4) producing S. enterica from a systemically unwell cat and three additional cats in the same facility. We have also performed for the first time a complete characterization of an IncHI2 plasmid heavy ...
A bifunctional ELP-MerR fusion protein is created to enable the precipitation of plasmid DNA, designed to contain the MerR recognition sequence, by a simple temperature trigger. The protocol covers all stages of the process from the design of ELP-MerR fusion proteins and MerR-binding plasmids, ...
Two most commonly used restriction endonulceases Hind III and EcoRI were used for digestion analysis of the extracted plasmid DNA samples as per the following protocol (Banglore GeNei Kit). λ /MuI molecular weight marker was also run parallel on agarose gel electrophore...