Jaoua S., Letouvet-Pawlak B., Monnier C., Guespin-Michel J. F. (1990). Mechanism of integration of the broad-host-range plasmid RP4 into the chromosome of Myxococcus xanthus . Plasmid 23 , 183–193 10.1016/0147-619X(90)90050-M [ Cross Ref ]...
(iii) Yeast integrating plasmids (YIp) are maintained by integration into a chromosome via homologous recombination. Most currently used yeast plasmids are based on pBluescript or pUC19 plasmids3,4 and can therefore replicate in both yeast and Escherichia coli. In addition to an AmpR gene for ...
Integration of the plasmid prophages P1 and P7 into the chromosome of Escherichia coli J. Mol. Biol. 130:161-173.Chesney, P. H., J. R. Scott, and D. Vapnek. 1979. Integration of the plasmid prophages P1 and P7 into the chromosome of Escherichia coli. J. Mol. Biol. 130:161-173....
Ohtsubo E, Hsu MT (1978) Electron microscope heteroduplex studies of sequence relations among plasmids ofE. coli: isolation of a new F-prime factor, F80, and its implication for the mechanism of F integration into the chromosome. J Bacteriol 134: 795–800 PubMedCASGoogle Scholar Ou JT (1...
A copy of the element is close (within 5 kb) of a gene coding for an extracellular agarase in the chromosome ofS. coelicolorA3(2) and in an NF strain, in which SCP1 has integrated into the chromosome, the agarase gene has been deleted. The element has properties reminiscent of Insertion...
Vir region –this region consists of virulence genes. It is responsible for the excision, integration, and transfer of T-DNA into the plantchromosome. Opine catabolism region –this region catabolizes opines, which are specialized amino acids. It is responsible for catabolizing opines produced by...
Most expression vectors for extrachromosomal protein expression and shuttle vectors contain the pBR322origin of replication, and fragments of pBR322 are very popular in the construction of intraspecies shuttle or binary vectors and vectors for targeted integration and excision of DNA from chromosome. ...
Interestingly, it appeared that the multi-copy genes integration into the chromosome, even at low copy number of XR genes like that of 2 or 4, could effectively lead to sufficient accumulation of xylitol. In addition to gene integration, the method used in this study allows for the insertion...
Development of a new integration site within the Bacillus subtilis chromosome and construction of compatible expression cassettes. J. Bacteriol. 183, 2696 (2001). Article PubMed PubMed Central Google Scholar Phan, T. T., Tran, L. T., Schumann, W. & Nguyen, H. D. Development of Pgrac...
The first step of allelic exchange was selection of plasmid integration into the recipient chromosome (which does not carry the pir gene) by plating cells on chloramphenicol-LB plates. For conjugation experiments, the donor strain SM10 λpir was eliminated by adding streptomycin in the medium. Aft...