A plasmid containing Fob1‐binding sites, when propagated in a yeast strain lacking is integrated into the yeast chromosome in a ‐dependent manner. We show that addition of nicotinamide (NAM) to the culture medium can stimulate such plasmid integration in the presence of . Pulsed‐field gel ...
(iii) Yeast integrating plasmids (YIp) are maintained by integration into a chromosome via homologous recombination. Most currently used yeast plasmids are based on pBluescript or pUC19 plasmids3,4 and can therefore replicate in both yeast and Escherichia coli. In addition to an AmpR gene for ...
Integration of the plasmid prophage P1 and P7 into the chromosome of Escherichia coli. J. Mol. Biol. 130:161-173.Chesney, RH, Scott, JR, Vapnek, D (1979) Integration of the plasmid prophages P1 and P7 into the chromosome of Escherichia coli. J Mol Biol 130: pp. 161-173...
and endogenous plasmids may play role in the evolution of this process. A plausible scenario is that denitrification genes are acquired by HGT and then become stabilised by integration into the chromosome, although even in the bona fide denitrifying strains IL106 and 2.4.3, some of the denitrific...
This has homologous sequences to the chromosome and after transformation, by recombination, it may be inserted in multiple copies into the yeast chromosome. Linearization of the plasmid may increase...Springer NetherlandsEncyclopedia of Genetics Genomics Proteomics & Informatics...
The frequency of plasmid pCET integration into the chromosome was approximately 10(-1) for all Bac. anthracis strains studied. In population of vegetative cells of strains Bac. anthracis (pCET), which passed through the sporulation stage under selective for plasmid pCET conditions, DNA of ...
Ohtsubo E, Hsu MT (1978) Electron microscope heteroduplex studies of sequence relations among plasmids ofE. coli: isolation of a new F-prime factor, F80, and its implication for the mechanism of F integration into the chromosome. J Bacteriol 134: 795–800 ...
The lambdoid phage N15 of Escherichia coli is very unusual among temperate phages in that its prophage is not integrated into chromosome but is a linear pl... Nikolai,V.,Ravin - 《Plasmid》 被引量: 84发表: 2011年 Genetic and physical map of a P1 miniplasmid The prophage form of bacteriop...
integrationintothechromosomeoccursvia homologousrecombination(Foster,1998).The productsofsinglecross-overrecombination,mero- diploidcellsharboringbothwild-typeandmutant alleles,areresolvedunderpermissiveconditions,in whichrecombinationandplasmidexcisionare thoughttobestimulatedbyrollingcirclereplica- tionoftheplasmid(Foster...
virus (HIV) genome into the host genome by preventing the suicidal autointegration to the viral genome as named after barrier-to-autointegration factor21,22. In addition, a recent report showed that BAF protects against the cGAS-STING response to prevent innate immune activation77,78. ...