Rheological properties of chromosomal and plasmid DNA during alkaline lysis reaction. Bioprocess Eng. 1999; 21 :231–237. doi: 10.1007/s004490050669. [ Cross Ref ]Ciccolini LAS, Ayazi Shamlou P, Titchener-Hooker NJ, Ward JM & Dunnill P (1999) Rheological properties of chromosomal and plasmid ...
Relatedness of Chromosomal and Plasmid DNAs of Erwinia pyrifoliae and Erwinia amylovora. Examines the divergence of the 16S-23S rRNA operon between Erwinia amylovora and Erwinia pyrifoliae and the organization of their plasmids. Origin of repli... McGhee,C Gayle,Schnabel,... - 《Applied & ...
Restriction endonuclease ( Eco RI, Bst EII, Pst I, and Pvu I) digests of chromosomal DNA were analysed using DNA probes encoding all or part of the... DL Williams,TP Gillis,F Portaels - 《Molecular Microbiology》 被引量: 89发表: 1990年 Restriction/modification in Streptococcus thermophilus:...
Plasmid DNA is defined as circular double-stranded DNA that serves as a gene-transfer vector, allowing the insertion of therapeutic transgenes with no size limit. It is easy to construct, propagate, and has a large packaging capacity, making it stable and characterized by low risk of insertiona...
The use of plasmid DNA (pDNA) as a pharmaceutical tool has increased since it represents a safer vector for gene transfer compared to viral vectors. Differ
, this purification scheme is performed in a few easy steps. First, in the cell lysis step, plasmid DNA is isolated from chromosomal DNA and cell debris. Next, plasmid DNA is bound to the PlasmidPURE spin filter and washed to remove any impurities. Finally, the plasmid DNA is eluted ...
What is the difference between plasmid DNA and chromosomal DNA? How did the discovery of the enzyme DNA polymerase play in the understanding for the process of DNA replication? How to clone a gene such as GFP into an E. coli cell?
A rapid and relatively simple procedure for purifying large quantities of plasmid DNA is described. Plasmid thus purified contains no detectable chromosomal DNA and little RNA or protein. The procedure combines alkaline denaturation and hydroxylapatite chromatography and utilizes an improved method of separ...
The methylation pattern of the plasmid DNA remained in its bacterial form indicating that the foreign DNA did not replicate in the muscle cells. The electroporation of total cellular DNA from injected muscles into bacteria indicated that the plasmid DNA was extrachromosomal. Chromosomal integration of ...
2.1Plasmid DNA Plasmid DNA(pDNA) is adouble stranded DNAwith a size ranging from hundreds to thousands bases assembled in a circular shape[26]. pDNA vectors carry a transgene which is replicated independently from host chromosomal DNA and segregated to each daughter cell resulting from cell divis...