1991. Cross hybridization of plasmid and genomic DNA from aromatic and polycyclic aromatic hydrocarbon degrading bacteria. Can. J. Microbiol. 37: 924-932.Foght JM, Westlake DWS (1991) Cross hybridization of plasmid and genomic DNA from aromatic and polycyclic aromatic hydrocarbon degrading bacteria....
However, for lab-scale preparative work, our findings show that plasmid yield can be significantly increased by using standard growth procedures and commonly used growth media. 展开 关键词: DH5α DNA EcoRI HindIII BamH1 plasmid 年份: 2021 ...
The gel-purified genomic DNA is then assayed for integrated plasmid using PCR. Stringent methods are used to prevent contamination. The assay, validated using a variety of positive and negative controls, is capable of detecting one copy of plasmid per ug DNA (approximately 150,000 diploid cells)...
Kumar, A, Bhawsar, NG, Badnagre, P, Panse, U, Gayakwad, SR, Khasdeo, K (2013) Isolation of Agrobacterium tumefaciens from soil and Optimization of Genomic & Plasmid DNA Extraction. Int J Adv Res 1: pp. 1-4Kumar A, Bhawsar NG, Badnagre P, Panse U, Gayakwad SR, Khasdeo K ...
, in Post-Genomic Cardiology (Second Edition), 2014 Naked plasmid DNA Nonviral vectors include naked plasmid DNA and DNA complexed with various macromolecules, such as polycationic lipids, polyethyleneimine, polylysine and polysaccharides.10,13,14 Plasmid DNA is the simplest gene-transfer vector, ...
Genomic DNA was extracted using Qiagen genomic tip 500/G kit and sequenced with PacBio RSII (Pacific Biosciences) using one SMRTMcell per isolate. For detailed data on the dataset see Supplementary TableS4. Hierarchical Genome Assembly Process (HGAP) was used to generate draft assemblies/genomes...
Using this assay, all exon-exon junctions of copyDNA of doping genes could be detected with a sensitivity of 1296 copyDNA copies in 1000ng of genomic DNA. In addition, promotor regions and plasmid-derived sequences are readily detectable in our sequence data. While we show the reliability of...
Notably, genomic DNA and mecA gene signals were more readily reduced by chlorine than the plasmid-borne tetA gene (by ~2 fold). Based on the results of qPCR with short (~200 bps) and long amplicons (~1200 bps), chlorination could destroy the integrity of ARGs, which likely reduces the ...
Restriction AnalysisEnzyme digestion and electrophoresisDNA bands with expected sizes detected by agarose gel electrophoresis Residual RNAVisual inspection upon electrophoresisNot visible on agarose gel E.coli Genomic DNA3, 5Visual inspection upon electrophoresis< 15% by agarose gel electrophoresis ...
Dos and Don’ts of Plasmid Minipreps Usage Guidelines for the Monarch Plasmid Miniprep Kit (#T1010) When Working with Low Copy Plasmids 应用实例 Using Exonuclease V (RecBCD) to Eliminate Residual Genomic DNA When Purifying Low Copy Plasmids with the Monarch®Plasmid Miniprep Kit ...