[0073]SQSTM1 cDNA(NM_003900)和缺失LIR的结构域的SQSTM1ΔLIR cDNA(图3A)分别克隆入pCMV‑HA质粒上的EcoRI/XhoI酶切位点之间,其中HA标签序列(DNA序列:TACCCATACGACGTCCCAGACTACGCT,编码氨基酸序列:YPYDVPDYA)位于多克隆位点上游,在大肠杆菌中扩增后提取质粒DNA,质粒DNA用Plasmid Midi kits(12145,Qiagen)提取...
Endotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin fibroblasts, primary human melanoma cells) in the prese...
Transient transfection of cells with miRNA mimic, short hairpin RNA (shRNA) or DNA plasmids was performed in 24-well plates using Lipofectamine 3000 reagent52(Life Technologies). For lnc-mg functional analyses, lnc-mg expression plasmid (500 ng per well) or empty plasmid (500 ng per well...
To visualize transfected DNA and its expression, we generated the following experimental system. We constructed a non-viral DNA plasmid carrying alacOrepeat sequence (256 repeats, approximately 10 kbp) and a sequence expressing monomeric red fluorescent protein (RFP) driven by the EF1α promoter (pL...
产品型号:ISRE luciferase reporter plasmid 产品展商:HZbscience 产品文档:无相关文档 简单介绍 ISRE luciferase reporter plasmid (ISRE-Luc荧光素酶报告基因质粒)用于检测ISRE转录活性水平为目的的报告基因。ISRE luciferase reporter plasmid (ISRE-Luc荧光素酶报告基因质粒)主要用于检测干扰素诱导的信号转导通路在细...
(C) Small RNA-seq of a heterologously expressed CRISPR-associated IscB locus (top) and RNP pulldown (bottom). (D) Sequence logo for the PAM as determined by a plasmid depletion assay. (E) In vitro cleavage by IscB-si...
AngularPlasmid is a DNA plasmid visualization component developed using Google's AngularJS framework. The project provides a set of Angular directives to allow for a declarative way to contruct a plasmid graph. Rather than coding client-side Javascript or other server-side programming languages, Angul...
Indeed, evidence is provided for a relationship between the arlRS deletion and topological changes in plasmid DNA. 展开 关键词: Staphylococcus aureus Novobiocin DNA Gyrase DNA, Bacterial DNA, Superhelical Bacterial Proteins Staphylococcal Protein A Protein Kinases Enzyme Inhibitors Virulence Factors DOI: ...
B and C, subcellular localization of FAM173A-derived GFP-fusion proteins. B, a schematic representation of the FAM173A-derived sequences used in (C), with the various domains denoted as in (A). C, confocal fluorescence microscopy images of live HeLa cells, stably transfected with plasmid ...
DNA assembly, respectively. Finally, we integrated the DNA assembly method and the software with a robotic system named Illinois Biological Foundry for Advanced Biomanufatcuring (iBioFAB) (Supplementary Fig.1) to create a nearly fully automated workflow for PlasmidMaker. As a proof of concept, ...