1991. Cross hybridization of plasmid and genomic DNA from aromatic and polycyclic aromatic hydrocarbon degrading bacteria. Can. J. Microbiol. 37:924-932.Foght JM, Westlake DWS (1991) Cross hybridization of plasmid and genomic DNA from aromatic and polycyclic aromatic hydrocarbon degrading bacteria. ...
Approaches to direct solid phase sequencing of genomic and plasmid DNA have been developed using magnetic beads, coated with streptavidin, as solid support. The DNA is immobilized through selective incorporation of biotin into one of the strands. A single stranded template, suitable for sequencing, ...
Isolation of Agrobacterium tumefaciens from soil and Optimization of Genomic and Plasmid DNA Extraction. International Journal of Advanced Research, 1(2): 1-4.Anil Kumar, Niharika, G, Bhawsar, Poornima Badnagre, Ujjaval Panse, SR, Gayakwad, and Krishna Khasdeo, (2013a). Isolation of ...
Pannucci J, Okinaka RT, Williams E, Sabin R, Ticknor LO, Kuske CR. DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria. BMC Genomics 2002;3:34.Pannucci J, Okinaka RT, Williams E, Sabin R, Ticknor LO, Kuske CR (2002...
The gel-purified genomic DNA is then assayed for integrated plasmid using PCR. Stringent methods are used to prevent contamination. The assay, validated using a variety of positive and negative controls, is capable of detecting one copy of plasmid per ug DNA (approximately 150,000 diploid cells)...
Genomic DNA was extracted using Qiagen genomic tip 500/G kit and sequenced with PacBio RSII (Pacific Biosciences) using one SMRTMcell per isolate. For detailed data on the dataset see Supplementary TableS4. Hierarchical Genome Assembly Process (HGAP) was used to generate draft assemblies/genomes...
Approaches to direct solid phase sequencing of genomic and plasmid DNA have been developed using magnetic beads, coated with streptavidin, as solid support... H Thomas,S Stefan,H Erik,... - 《Nucleic Acids Research》 被引量: 1592发表: 1989年 Cloning, sequencing, and expression in Escherichia...
Concentration and quality of the extracted plasmid and genomic DNA was assessed using the NanoDrop™ (Thermo Fisher Scientific) and Qubit (Invitrogen by Life Technologies). The sample libraries for Illumina sequencing were prepared using the Nextera Flex library kit (Illumina, Inc) and sequencing ...
Genomic DNA (gDNA) and plasmid DNA (pDNA) was extracted from culturedB.bigeminaandB.bovisusing the Qiagen Blood core kit according to the manufacturer’s instructions. Plasmid constructs The 3′ and 5′ insertion target regions for stable transfection in theef-1-alpha-Borf were generated by PC...
As of October 2006, the repository contains >46 000 plasmids in 98 different vectors, including cloned cDNA and genomic fragments from 26 different species. Moreover, the clones include plasmid vectors useful for routine and cutting-edge techniques; functionally related sets of human cDNA clones; ...